Scholarlink

<p>The archaeon <i>Methanococcus maripaludis</i> (<i>M. maripaludis</i>) is a model organism for studying archaeal physiology and energy conservation in the hydrogenotrophic methanogenesis pathway. <i>M. maripaludis</i> has a distinct proteome allocation strategy, in which ribosomal proteome allocations do not change with growth rates. Here, we developed a proteome-constrained metabolic model that can explain this different proteome allocation strategy. First, we used multiple bioinformatics databases to compile information about the translational process and enzymatic complexes. We then extended a genome-scale metabolic model of <i>M. maripaludis</i> with protein synthesis processes, including ribosome assembly, tRNA charging, and enzyme assembly reactions. The proposed model predicts alternative proteome resource allocation strategies and mutant fitness for this archaeon under different conditions. Therefore, our model provides a framework for studying the effects of resource allocation on the hydrogenotrophic methanogenesis pathway.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Whole-cell modeling predicts alternative proteome allocation strategies in the archaeon Methanococcus maripaludis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月19日

查看全文
  • Ghada S. Kasem,
  • Taysir Hassan A. Soliman,
  • Mohamed A. Ali Mousa,
  • Zeinhum F. Jaheen,
  • Ibrahim E. Elsemman

摘要

The archaeon Methanococcus maripaludis (M. maripaludis) is a model organism for studying archaeal physiology and energy conservation in the hydrogenotrophic methanogenesis pathway. M. maripaludis has a distinct proteome allocation strategy, in which ribosomal proteome allocations do not change with growth rates. Here, we developed a proteome-constrained metabolic model that can explain this different proteome allocation strategy. First, we used multiple bioinformatics databases to compile information about the translational process and enzymatic complexes. We then extended a genome-scale metabolic model of M. maripaludis with protein synthesis processes, including ribosome assembly, tRNA charging, and enzyme assembly reactions. The proposed model predicts alternative proteome resource allocation strategies and mutant fitness for this archaeon under different conditions. Therefore, our model provides a framework for studying the effects of resource allocation on the hydrogenotrophic methanogenesis pathway.