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<p>Crosslinking strengthens the sclera and holds potential as a treatment for myopia. This study aims to identify optimal crosslinking parameters in guinea pigs using WST11 with dextran followed by near-infrared (NIR) illumination. Guinea pig eyes were incubated in WST11 with 2, 5 or 10% dextran, and penetration depth was assessed by fluorescence microscopy. Crosslinking efficacy was measured as thermal stability using a thermal degradation assay, following incubation in WST11 + 10% dextran (WST-D) for 30&#xa0;min and NIR irradiation at 10 mW/cm<sup>2</sup> or 20 mW/cm<sup>2</sup> for 10, 20 and 30&#xa0;min. The optimized parameters were then applied in vivo in 6-month-old guinea pigs. <i>Ex vivo</i> treatment using the optimal crosslinking parameters (WST-D, 30&#xa0;min; NIR, 10 mW/cm<sup>2</sup>, 30&#xa0;min) resulted in the highest thermal degradation midpoint (<i>ΔT</i><sub><i>50</i></sub>: 6.8), significantly higher than untreated controls (<i>p</i> = 0.0006), with WST-D penetration limited to the sclera. Efficacy was greater in eyes obtained from older compared to younger guinea pigs (<i>p</i> = 0.02). <i>In vivo</i>, WST-D/NIR treatment resulted in significant crosslinking compared to untreated controls (equatorial, <i>ΔT</i><sub><i>50</i></sub>: 3.7, <i>p</i> &lt; 0.0001; posterior, <i>ΔT</i><sub><i>50</i></sub>: 3.4, <i>p</i> = 0.01). WST-D/NIR treatment effectively induces scleral crosslinking, with age-related differences suggesting the need for personalized treatment.</p>

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A transpupillary approach for crosslinking Guinea pig sclera using WST11 and near-infrared light

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月24日

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  • Demi H. J. Vogels,
  • Yusupjan Abdulla,
  • William Myles,
  • Sara Cummings,
  • Lilach Agemy,
  • Tamar Yechezkel,
  • Arie L. Marcovich,
  • Avigdor Scherz,
  • Vanessa L. S. LaPointe,
  • Sally A. McFadden,
  • Mor M. Dickman

摘要

Crosslinking strengthens the sclera and holds potential as a treatment for myopia. This study aims to identify optimal crosslinking parameters in guinea pigs using WST11 with dextran followed by near-infrared (NIR) illumination. Guinea pig eyes were incubated in WST11 with 2, 5 or 10% dextran, and penetration depth was assessed by fluorescence microscopy. Crosslinking efficacy was measured as thermal stability using a thermal degradation assay, following incubation in WST11 + 10% dextran (WST-D) for 30 min and NIR irradiation at 10 mW/cm2 or 20 mW/cm2 for 10, 20 and 30 min. The optimized parameters were then applied in vivo in 6-month-old guinea pigs. Ex vivo treatment using the optimal crosslinking parameters (WST-D, 30 min; NIR, 10 mW/cm2, 30 min) resulted in the highest thermal degradation midpoint (ΔT50: 6.8), significantly higher than untreated controls (p = 0.0006), with WST-D penetration limited to the sclera. Efficacy was greater in eyes obtained from older compared to younger guinea pigs (p = 0.02). In vivo, WST-D/NIR treatment resulted in significant crosslinking compared to untreated controls (equatorial, ΔT50: 3.7, p < 0.0001; posterior, ΔT50: 3.4, p = 0.01). WST-D/NIR treatment effectively induces scleral crosslinking, with age-related differences suggesting the need for personalized treatment.