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<p><i>Gibellula scorpioides</i> BCC 39989 is one of the species of spider-parasitic <i>Gibellula</i> (<i>Hypocreales</i>,<i> Cordycipitaceae</i>) discovered in Thailand. Investigation of the invertebrate-pathogenic fungus <i>G. scorpioides</i> BCC 39989 led to the isolation of eight known compounds (<b>1</b>–<b>8</b>). Their chemical structures were determined by employing extensive spectroscopic analysis. Among them, ergosterol peroxide (<b>1</b>) and methyl <i>o</i>-hydroxycinnamate (<b>2</b>) exhibited significant biological activities. Both compounds notably suppressed LPS-induced nitric oxide production and reduced IL-6 and TNF-α secretion in RAW264.7 macrophages. In addition, compounds <b>1</b>, <b>2</b>, and oleic acid (<b>7</b>) significantly inhibited lipid accumulation and reduced intracellular triglyceride content in 3T3-L1 adipocytes in a dose-dependent manner. Compound <b>1</b> also displayed potent antiproliferative activity against human colorectal adenocarcinoma (SW480) cells, with an IC<sub>50</sub> value of 13.33&#xa0;µg/mL, whereas compound <b>2</b> was less effective. Genotoxicity evaluation of ergosterol peroxide (<b>1</b>) using the cytokinesis-block micronucleus assay in V79 cells revealed no genotoxic effects at concentrations ranging from 3.125 to 50&#xa0;µg/mL, both with and without metabolic activation. These findings highlight <i>G. scorpioides</i> as a promising source of bioactive compounds, particularly ergosterol peroxide (<b>1</b>), which exhibits potent anti-inflammatory, anti-adipogenic, and antiproliferative properties without genotoxicity.</p>

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Chemical and biological profiling of bioactive metabolites from the invertebrate-pathogenic fungus Gibellula scorpioides BCC 39989

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月9日

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  • Ubon Rerk-am,
  • Boonchoy Soontornworajit,
  • Yuthana Tantirungrotechai,
  • Pichayanoot Rotkrua,
  • Praphakorn Kaemchantuek,
  • Sarunya Laovitthayanggoon,
  • Pattarawadee Kengkwasingh,
  • Artit Khonsanit,
  • Donnaya Thanakitpipattana,
  • Wilawan Kuephadungphan,
  • Janet Jennifer Luangsa-ard,
  • Jiraporn Arunpanichlert

摘要

Gibellula scorpioides BCC 39989 is one of the species of spider-parasitic Gibellula (Hypocreales, Cordycipitaceae) discovered in Thailand. Investigation of the invertebrate-pathogenic fungus G. scorpioides BCC 39989 led to the isolation of eight known compounds (1–8). Their chemical structures were determined by employing extensive spectroscopic analysis. Among them, ergosterol peroxide (1) and methyl o-hydroxycinnamate (2) exhibited significant biological activities. Both compounds notably suppressed LPS-induced nitric oxide production and reduced IL-6 and TNF-α secretion in RAW264.7 macrophages. In addition, compounds 1, 2, and oleic acid (7) significantly inhibited lipid accumulation and reduced intracellular triglyceride content in 3T3-L1 adipocytes in a dose-dependent manner. Compound 1 also displayed potent antiproliferative activity against human colorectal adenocarcinoma (SW480) cells, with an IC50 value of 13.33 µg/mL, whereas compound 2 was less effective. Genotoxicity evaluation of ergosterol peroxide (1) using the cytokinesis-block micronucleus assay in V79 cells revealed no genotoxic effects at concentrations ranging from 3.125 to 50 µg/mL, both with and without metabolic activation. These findings highlight G. scorpioides as a promising source of bioactive compounds, particularly ergosterol peroxide (1), which exhibits potent anti-inflammatory, anti-adipogenic, and antiproliferative properties without genotoxicity.