Scholarlink

<p>Industrial applications are evolving to replace chemical catalysts with eco-friendly solutions. This work enhances extracellular protease production from a newly isolated proteolytic <i>Bacillus paramycoides</i> WSA using Response Surface Methodology (RSM). The used quadratic model demonstrated a good fit with R<sup>2</sup> = 80.59%, R<sup>2</sup><sub>Adj</sub> = 57.94%, a high degree of significance (<i>p</i> = 0.017 &lt; 0.05), and a low RMSE = 0.030 U/mL. Maximum protease activity of 1.346 ± 0.060 U/mL was obtained after 8&#xa0;h-cultivation at 45&#xa0;°C and pH 7. The protease activity is maximal at 45&#xa0;°C and pH 10. It maintains 75% of its activity after 10&#xa0;min of incubation at 80&#xa0;°C. Furthermore, the enzyme was found to be stable in an alkaline environment, retaining 89% of its activity after 1&#xa0;h incubation at pH 12. Several metal ions, surfactants, and inhibitors increased protease performance. Indeed, 110% and 140% protease activity was measured in the presence of 5 mM CoCl<sub>2</sub> and CaCl<sub>2</sub>, respectively. Similarly, 105%, 128%, 135%, 144%, and 160% protease activity were measured in the presence of 0.5% of Tween 20, EDTA, Sodium azide, uric acid, and SDS, respectively. Interestingly, the enzyme activity was enhanced (138%) after 1&#xa0;h of incubation with 1% Reem detergent. Furthermore, this protease (10 U/mL), in combination with 0.7% detergent, could clean bloodstained fabric after 10&#xa0;min incubation at 45&#xa0;°C. Our results suggest that this thermostable, alkaline, and detergent-biocompatible protease could be a promising additive for eco-friendly use in detergent industries.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Production optimization of a thermostable alkaline and detergent biocompatible protease by Bacillus paramycoides WSA for the green detergent industry

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月16日

查看全文
  • Wafa A. Alshehri,
  • Sarah A. Alhothifi,
  • Ashjan F. Khalel,
  • Fatimah S. Alqahtani,
  • Bilel Hadrich,
  • Adel Sayari

摘要

Industrial applications are evolving to replace chemical catalysts with eco-friendly solutions. This work enhances extracellular protease production from a newly isolated proteolytic Bacillus paramycoides WSA using Response Surface Methodology (RSM). The used quadratic model demonstrated a good fit with R2 = 80.59%, R2Adj = 57.94%, a high degree of significance (p = 0.017 < 0.05), and a low RMSE = 0.030 U/mL. Maximum protease activity of 1.346 ± 0.060 U/mL was obtained after 8 h-cultivation at 45 °C and pH 7. The protease activity is maximal at 45 °C and pH 10. It maintains 75% of its activity after 10 min of incubation at 80 °C. Furthermore, the enzyme was found to be stable in an alkaline environment, retaining 89% of its activity after 1 h incubation at pH 12. Several metal ions, surfactants, and inhibitors increased protease performance. Indeed, 110% and 140% protease activity was measured in the presence of 5 mM CoCl2 and CaCl2, respectively. Similarly, 105%, 128%, 135%, 144%, and 160% protease activity were measured in the presence of 0.5% of Tween 20, EDTA, Sodium azide, uric acid, and SDS, respectively. Interestingly, the enzyme activity was enhanced (138%) after 1 h of incubation with 1% Reem detergent. Furthermore, this protease (10 U/mL), in combination with 0.7% detergent, could clean bloodstained fabric after 10 min incubation at 45 °C. Our results suggest that this thermostable, alkaline, and detergent-biocompatible protease could be a promising additive for eco-friendly use in detergent industries.