Scholarlink

<p>Acute febrile illness (AFI) investigations are crucial for public health. They can provide data on disease prevalence, morbidity, and mortality, and improve treatment, management, control, and detection of outbreaks in areas with limited diagnostic tests. Current understanding of multiple causes of AFI in the paediatric population in Tanzania is limited. This study aimed to simultaneously detect 33 pathogens using TaqMan Array Card based real-time PCR. Whole blood samples were collected from a total of 247 children (2–59 months old) who presented with febrile illness at Dareda and Haydom hospitals in north-eastern Tanzania between November 2015 and March 2016. Overall, 50 (20.2%) and 8 (3.2%) of 247&#xa0;children had at least one and more than one pathogen detected respectively. Bacterial zoonoses were frequently detected including <i>Brucella</i> spp. (<i>n</i> = 18, 7.3%), <i>C. burnetii</i> (<i>n</i> = 4, 1.6%), <i>Bartonella</i> spp. (<i>n</i> = 3, 1.2%), <i>Rickettsia</i> spp. (<i>n</i> = 3, 1.2%) and <i>Leptospira</i> spp. (<i>n</i> = 1, 0.4%). Dengue virus was detected in 14 (5.7%) individuals and <i>Plasmodium</i> spp. in 12 (4.9%) individuals. These findings reveal the potential clinical importance of zoonoses and arboviruses in febrile children in Tanzania and highlight the need to consider a broad range of pathogens in febrile illness diagnosis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Detection of pathogens associated with acute febrile illness in children under five years of age in rural Tanzania

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月4日

查看全文
  • Athanasia Maro,
  • AbdulHamid S. Lukambagire,
  • Blandina T. Mmbaga,
  • Jie Liu,
  • Sixbert I. Mkumbaye,
  • Nelson Amani,
  • Judith Njau,
  • Reginald A. Kavishe,
  • Jean Gratz,
  • Eric R. Houpt,
  • Jo E. B. Halliday

摘要

Acute febrile illness (AFI) investigations are crucial for public health. They can provide data on disease prevalence, morbidity, and mortality, and improve treatment, management, control, and detection of outbreaks in areas with limited diagnostic tests. Current understanding of multiple causes of AFI in the paediatric population in Tanzania is limited. This study aimed to simultaneously detect 33 pathogens using TaqMan Array Card based real-time PCR. Whole blood samples were collected from a total of 247 children (2–59 months old) who presented with febrile illness at Dareda and Haydom hospitals in north-eastern Tanzania between November 2015 and March 2016. Overall, 50 (20.2%) and 8 (3.2%) of 247 children had at least one and more than one pathogen detected respectively. Bacterial zoonoses were frequently detected including Brucella spp. (n = 18, 7.3%), C. burnetii (n = 4, 1.6%), Bartonella spp. (n = 3, 1.2%), Rickettsia spp. (n = 3, 1.2%) and Leptospira spp. (n = 1, 0.4%). Dengue virus was detected in 14 (5.7%) individuals and Plasmodium spp. in 12 (4.9%) individuals. These findings reveal the potential clinical importance of zoonoses and arboviruses in febrile children in Tanzania and highlight the need to consider a broad range of pathogens in febrile illness diagnosis.