Scholarlink

<p>Increasing water scarcity, driven by population growth and climate change, necessitates the development of biomanufacturing processes that maximize water-use efficiency. This report illustrates the potential of biocatalyst and supernatant recycling in a decoupled bioprocess integrated with product removal systems for <i>trans</i>-cinnamic production using <i>Pseudomonas putida</i> KT2440 expressing RmXAL. With a biocatalyst concentration of 40 g<sub>DCW</sub> L<sup>− 1</sup> and an L-phenylalanine feed concentration of 35 g<sub>L−phe</sub> L<sup>− 1</sup> combined with product precipitation, the process achieved a titer of 143.00&#xa0;g L<sup>− 1</sup>, a production rate of 2.25&#xa0;g L<sup>− 1</sup> h<sup>− 1</sup>, a yield of 1.50&#xa0;g <i>t</i>CA g glucose<sup>− 1</sup>, and a yield on L-phenylalanine of 0.64&#xa0;g <i>t</i>CA g L-phe<sup>− 1</sup>. Furthermore, this optimized approach substantially reduced water consumption and wastewater generation by 85% compared to standard production processes, promoting the principles of Sustainable Development Goal 6 (SDG) “Clean water and sanitation” and SDG 12 “Responsible production and consumption” thus enabling an industrial bioproduction platform to achieve better environmental and economic sustainability.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

High-efficiency water use and process performance for bioproduction of trans-cinnamic acid

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月4日

查看全文
  • Sompot Antimanon,
  • Sheila Ingemann Jensen,
  • John M. Woodley

摘要

Increasing water scarcity, driven by population growth and climate change, necessitates the development of biomanufacturing processes that maximize water-use efficiency. This report illustrates the potential of biocatalyst and supernatant recycling in a decoupled bioprocess integrated with product removal systems for trans-cinnamic production using Pseudomonas putida KT2440 expressing RmXAL. With a biocatalyst concentration of 40 gDCW L− 1 and an L-phenylalanine feed concentration of 35 gL−phe L− 1 combined with product precipitation, the process achieved a titer of 143.00 g L− 1, a production rate of 2.25 g L− 1 h− 1, a yield of 1.50 g tCA g glucose− 1, and a yield on L-phenylalanine of 0.64 g tCA g L-phe− 1. Furthermore, this optimized approach substantially reduced water consumption and wastewater generation by 85% compared to standard production processes, promoting the principles of Sustainable Development Goal 6 (SDG) “Clean water and sanitation” and SDG 12 “Responsible production and consumption” thus enabling an industrial bioproduction platform to achieve better environmental and economic sustainability.