Scholarlink

<p>Coagulation test results are influenced by many pre-analytical variables, including clinical sample storage conditions. This study investigated the effects of storage time and temperature on coagulation factor (FII, FV, FVII, FX, FVIII, FIX, FXI, FXII, and FXIII) and natural anticoagulant (antithrombin, protein C, and free protein S) activities. Blood samples were collected from 78 healthy individuals, and 3 samples were used for each coagulation factor and natural anticoagulant. Plasma was tested after centrifugation and storage at room temperature (18 to 25&#xa0;°C) or refrigerated (2 to 8&#xa0;°C) for 2, 3, 4, and 5&#xa0;h, or frozen (− 15 to − 25&#xa0;°C) for 1, 2, 3, and 4 months. For all storage conditions, the mean percentage change from baseline in evaluated activities was &lt; 15%, except for FV and FVIII. For FV activities after frozen storage at − 15 to − 25&#xa0;°C and FVIII activities at all storage conditions, the mean percentage change was &gt; 15%. For FV and FVIII activities, the mean percentage change was &lt; 15% after frozen storage at − 75 to − 85&#xa0;°C for 1, 2, 3, and 4 months. To ensure appropriate clinical testing results, especially for FV and FVIII activity, storage condition and time should be critically controlled.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Effects of storage time and temperature on coagulation factor and natural anticoagulant activities in healthy individuals

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年3月28日

查看全文
  • Ryosuke Nakanishi,
  • Tasuku Sakayori,
  • Daichi Matsui,
  • Mai Kono,
  • Minase Maki,
  • Claire Dunois,
  • Yutaka Komiyama,
  • Hiromi Morisaki

摘要

Coagulation test results are influenced by many pre-analytical variables, including clinical sample storage conditions. This study investigated the effects of storage time and temperature on coagulation factor (FII, FV, FVII, FX, FVIII, FIX, FXI, FXII, and FXIII) and natural anticoagulant (antithrombin, protein C, and free protein S) activities. Blood samples were collected from 78 healthy individuals, and 3 samples were used for each coagulation factor and natural anticoagulant. Plasma was tested after centrifugation and storage at room temperature (18 to 25 °C) or refrigerated (2 to 8 °C) for 2, 3, 4, and 5 h, or frozen (− 15 to − 25 °C) for 1, 2, 3, and 4 months. For all storage conditions, the mean percentage change from baseline in evaluated activities was < 15%, except for FV and FVIII. For FV activities after frozen storage at − 15 to − 25 °C and FVIII activities at all storage conditions, the mean percentage change was > 15%. For FV and FVIII activities, the mean percentage change was < 15% after frozen storage at − 75 to − 85 °C for 1, 2, 3, and 4 months. To ensure appropriate clinical testing results, especially for FV and FVIII activity, storage condition and time should be critically controlled.