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<p>Subtilisin-like serine proteases (SBTs) are serine proteolytic enzymes that play various roles in plant growth, function and stress responses. <i>Vigna glabrescens</i>, a wild relative of mungbean known to be a potential donor of photo- and thermoperiod insensitivity, was characterized for thermotolerance through reproductive biology and gene expression profiling. Whole-genome sequencing of this species has not yet been performed; hence, genome-wide analysis of this species has not been explored. In the present study, a systematic analysis of SBT-encoding genes in the <i>V. radiata</i> (Vradi_SBT) genome was conducted, with a focus on their response during flower development under different temperature regimes, such as optimum temperature, heat and cold stresses, in <i>Vigna radiata</i> and a wild relative, <i>Vigna glabrescens</i>. Thirty-eight Vradi_SBT genes were identified in the <i>V. radiata</i> genome and were further grouped into five distinct subgroups. The key domain of the SBT peptidase, “peptidase_S8_53,” was found in all 38 Vradi_SBT proteins, while 28 of them contained the “peptidase_S8” domain. Additionally, 30 of these proteins contained a maximum of 10 motifs. A total of 22 orthologous genes were identified in <i>Arabidopsis thaliana</i>, whereas paralogous gene pairs were detected as tandemly duplicated genes with <i>V. unguiculata</i>. <i>Cis</i>-acting element analysis revealed that these genes presented more stress-responsive promoter sequences than the other promoters. Furthermore, <i>Vradi_SBT-</i>1.9 was found significantly upregulated under both high- and low-temperature stresses. This study provides insights into SBT-encoding genes and their possible role in flower development and thermotolerance in <i>Vigna</i> species.</p>

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Genome-wide identification and characterization of Subtilisin-like Serine protease encoding genes in Vigna radiata L. Wilczek

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月17日

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  • Poornima Singh,
  • Brijesh Pandey,
  • Shalini Purwar,
  • Ramakrishnan Madhavan Nair,
  • Aditya Pratap

摘要

Subtilisin-like serine proteases (SBTs) are serine proteolytic enzymes that play various roles in plant growth, function and stress responses. Vigna glabrescens, a wild relative of mungbean known to be a potential donor of photo- and thermoperiod insensitivity, was characterized for thermotolerance through reproductive biology and gene expression profiling. Whole-genome sequencing of this species has not yet been performed; hence, genome-wide analysis of this species has not been explored. In the present study, a systematic analysis of SBT-encoding genes in the V. radiata (Vradi_SBT) genome was conducted, with a focus on their response during flower development under different temperature regimes, such as optimum temperature, heat and cold stresses, in Vigna radiata and a wild relative, Vigna glabrescens. Thirty-eight Vradi_SBT genes were identified in the V. radiata genome and were further grouped into five distinct subgroups. The key domain of the SBT peptidase, “peptidase_S8_53,” was found in all 38 Vradi_SBT proteins, while 28 of them contained the “peptidase_S8” domain. Additionally, 30 of these proteins contained a maximum of 10 motifs. A total of 22 orthologous genes were identified in Arabidopsis thaliana, whereas paralogous gene pairs were detected as tandemly duplicated genes with V. unguiculata. Cis-acting element analysis revealed that these genes presented more stress-responsive promoter sequences than the other promoters. Furthermore, Vradi_SBT-1.9 was found significantly upregulated under both high- and low-temperature stresses. This study provides insights into SBT-encoding genes and their possible role in flower development and thermotolerance in Vigna species.