<p>Traditional villages, an important rural cultural heritage, have gradually developed tourism value and become attractive tourism resources with the support of increasingly convenient transportation infrastructure, and there is inevitably a correlation between the spatial pattern distribution and tourism attractiveness as well as transportation accessibility. Therefore, the study adopts ArcGIS, a modified gravity model combined with the research method of transportation accessibility, to investigate the interaction mechanism and influence relationship between 724 traditional villages in 88 districts and counties of Guizhou Province as the research object. The results found that: (1) Traditional villages in Guizhou Province are mainly clustered in Qiandongnan, with an overall clustering distribution. (2) Guizhou has formed an attraction structure centered on the central Guizhou economic circle. The number of traditional villages represents the richness of their cultural tourism resources, which affects the tourism economic links between regions. (3) Traditional villages are mainly centered on Qiandongnan, forming six isochronous circles. The regional superposition effect generated by transportation accessibility will accelerate the formation of the core advantages of tourist destinations. (4) The degree of agglomeration of traditional villages will affect the advantages and disadvantages of rural cultural tourism resource endowment. Transportation and tourism are mutually beneficial and coexistent, and when the roads are clear and not blocked, the industry will prosper, and when the scenery is beautiful, the people will be rich. The study provides ideas for deepening the integration of road and tourism in traditional villages, and is also activating the tourism value of traditional villages and promoting the layout of reverse and niche tourism products in rural areas.</p>