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<p><i>WNT16</i> stands up as an essential gene for bone homeostasis. Here, we present new evidence of the functional role of a particular region within <i>WNT16</i>. Performing 4&#xa0;C chromatin conformation analysis in three osteoblast-related cells (the human fetal osteoblast hFOB 1.19 cell line, Saos 2 osteosarcoma cell line and mesenchymal Stem Cells –MSC-), we identify physical interactions between the proximal part of <i>WNT16</i> intron 2, shown here to be an active promoter in Saos 2 osteosarcoma cells, and several putative regulatory regions within <i>CPED1</i>. Analysis of previously published RNA-seq data from hFOB cells disclosed low expression of a region located downstream of this promoter. Our results suggest a novel regulatory mechanism of <i>WNT16</i> in bone, mediated by physical interaction with various enhancer regions within <i>CPED1</i>.</p>

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Regulation of WNT16 in bone may involve upstream enhancers within CPED1

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月20日

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  • N. Martínez-Gil,
  • C. Herrera-Ubeda,
  • N. Gritti,
  • N. Roca-Ayats,
  • N. Ugartondo,
  • N. Garcia-Giralt,
  • D. Ovejero,
  • X. Nogués,
  • J. Garcia-Fernàndez,
  • Daniel Grinberg,
  • Susanna Balcells

摘要

WNT16 stands up as an essential gene for bone homeostasis. Here, we present new evidence of the functional role of a particular region within WNT16. Performing 4 C chromatin conformation analysis in three osteoblast-related cells (the human fetal osteoblast hFOB 1.19 cell line, Saos 2 osteosarcoma cell line and mesenchymal Stem Cells –MSC-), we identify physical interactions between the proximal part of WNT16 intron 2, shown here to be an active promoter in Saos 2 osteosarcoma cells, and several putative regulatory regions within CPED1. Analysis of previously published RNA-seq data from hFOB cells disclosed low expression of a region located downstream of this promoter. Our results suggest a novel regulatory mechanism of WNT16 in bone, mediated by physical interaction with various enhancer regions within CPED1.