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<p>In this study, a novel artificial enzyme Zn(II)-SMM with simple organic structure for PNPA-directed hydrolysis is proposed. The introduction of hydrophobic quinoline and dipyridinium in Zn(II)-SMM system demonstrates high activity for the complexation of Zn<sup>2+</sup> and hydrolysis of PNPA, and the catalytic rate (k<sub>cat</sub>/k<sub>uncat</sub>) exceeds 5239 times that of non-catalytic systems. The newly designed small molecule organic complex effectively binds to and catalyzes the hydrolysis of substrate PNPA in accordance with Michaelis-Menten kinetics. The mechanism with activation by single Lewis acid provides fundamental insights for the development of small molecule hydrolases.</p>

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A novel simplified structural design as an artificial enzyme for efficient hydrolysis of PNPA

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月17日

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  • Wenfang Li,
  • Yuze Lu,
  • Jiajun Wang,
  • Chuanbi Li

摘要

In this study, a novel artificial enzyme Zn(II)-SMM with simple organic structure for PNPA-directed hydrolysis is proposed. The introduction of hydrophobic quinoline and dipyridinium in Zn(II)-SMM system demonstrates high activity for the complexation of Zn2+ and hydrolysis of PNPA, and the catalytic rate (kcat/kuncat) exceeds 5239 times that of non-catalytic systems. The newly designed small molecule organic complex effectively binds to and catalyzes the hydrolysis of substrate PNPA in accordance with Michaelis-Menten kinetics. The mechanism with activation by single Lewis acid provides fundamental insights for the development of small molecule hydrolases.