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<p>Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) facilitate intracellular vesicle trafficking and membrane fusion in eukaryotic organisms, significantly influencing fungal growth, development, and pathogenicity. Despite their importance, the specific functions of individual SNARE subunits remain largely unexplored. In <i>Cytospora chrysosperma</i>, a pathogen causing substantial damage to woody plants and leading to considerable ecological and economic impacts, 22 SNAREs have been identified. Yet, their functional roles have not been previously characterized. In this study, we focused on two SNARE-encoding genes, <i>CcSec22</i> and <i>CcSso1</i> that are supposed to mediate endoplasmic reticulum (ER)-Golgi and trans-Golgi Network (TGN)-secretory vesicle transport respectively. Notably, both genes demonstrate significantly elevated expression during the infection process. Targeted deletion of either of them resulted in retarded mycelial growth and remarkably decreased tolerance towards different cell-wall stressors. Interestingly, <i>CcSec22</i> deletion mutants formed more hyphal branches while <i>CcSso1</i> was required for morphological maintenance of conidiospores. More importantly, lack of either of them significantly reduced hyphal endocytosis and fungal virulence on host poplar. Collectively, our data highlighted that the SNARE genes <i>CcSec22</i> and <i>CcSso1</i> play pleiotropic roles in mycelial growth and development, stress responses, conidiation, endocytosis and in particular, they are required for the full virulence of <i>C. chrysosperma</i>. This study provides an insight into the role of SNARE proteins in <i>C. chrysosperma</i> pathogenesis.</p>

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SNARE genes CcSec22 and CcSso1 coordinate fungal growth, sporulation, cell wall stress tolerance, endocytosis and full virulence in Cytospora chrysosperma

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月11日

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  • Zhu Han,
  • Zheng Luo,
  • Xueyan Li,
  • Dianguang Xiong,
  • Chengming Tian

摘要

Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) facilitate intracellular vesicle trafficking and membrane fusion in eukaryotic organisms, significantly influencing fungal growth, development, and pathogenicity. Despite their importance, the specific functions of individual SNARE subunits remain largely unexplored. In Cytospora chrysosperma, a pathogen causing substantial damage to woody plants and leading to considerable ecological and economic impacts, 22 SNAREs have been identified. Yet, their functional roles have not been previously characterized. In this study, we focused on two SNARE-encoding genes, CcSec22 and CcSso1 that are supposed to mediate endoplasmic reticulum (ER)-Golgi and trans-Golgi Network (TGN)-secretory vesicle transport respectively. Notably, both genes demonstrate significantly elevated expression during the infection process. Targeted deletion of either of them resulted in retarded mycelial growth and remarkably decreased tolerance towards different cell-wall stressors. Interestingly, CcSec22 deletion mutants formed more hyphal branches while CcSso1 was required for morphological maintenance of conidiospores. More importantly, lack of either of them significantly reduced hyphal endocytosis and fungal virulence on host poplar. Collectively, our data highlighted that the SNARE genes CcSec22 and CcSso1 play pleiotropic roles in mycelial growth and development, stress responses, conidiation, endocytosis and in particular, they are required for the full virulence of C. chrysosperma. This study provides an insight into the role of SNARE proteins in C. chrysosperma pathogenesis.