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<p>The evolution of genetic diversity and population structure of <i>Plasmodium vivax</i> as malaria elimination approaches remains unclear. This study analyzed the genetic variation and molecular epidemiology of <i>P. vivax</i> from Yala Province in southern Thailand, an area in the pre-elimination phase. Seventy <i>P. vivax</i> isolates, collected between 2017 and 2020, were genotyped for domain II of <i>pvdbp</i> and the 42-kDa region of <i>pvmsp1</i> using amplicon deep sequencing. Data from Yala province were compared to published data from Tak province, where transmission was higher. Key analyses included nucleotide diversity (π), haplotype diversity (Hd), natural selection, recombination rates, and complexity of infection (COI). Genetic diversity in Yala was relatively low (π = 0.008<sup>dbp</sup> and 0.014<sup>msp1</sup>; Hd = 0.774<sup>dbp</sup> and 0.407<sup>msp1</sup>) compared to Tak (π = 0.012<sup>dbp</sup> and 0.027<sup>msp1</sup>; Hd = 0.849<sup>dbp</sup> and 0.962<sup>msp1</sup>). In Yala, polyclonal infections were found in 53.7% of <i>pvdbp</i><sub><i>II</i></sub> and 47.8% of <i>pvmsp1</i><sub><i>42</i></sub> isolates, with average COI of 1.6 and 1.7. Both genes were under balancing selection. Distinct genetic differences were found between Yala and Tak in <i>pvmsp1</i><sub><i>42</i></sub>, providing a local genotypic profile useful for tracing parasite origins.</p>

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Molecular epidemiology and genetic diversity of disappearing Plasmodium vivax in southern Thailand

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月21日

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  • Parsakorn Tapaopong,
  • Gustavo da Silva,
  • Aurel Holzschuh,
  • Wasinee Rungsarityotin,
  • Chayanut Suansomjit,
  • Kanit Pumchuea,
  • Khajohnpong Manopwisedjaroen,
  • Amnat Khamsiriwatchara,
  • Podjadeach Khuntong,
  • Liwang Cui,
  • Cristian Koepfli,
  • Jetsumon Sattabongkot,
  • Wang Nguitragool

摘要

The evolution of genetic diversity and population structure of Plasmodium vivax as malaria elimination approaches remains unclear. This study analyzed the genetic variation and molecular epidemiology of P. vivax from Yala Province in southern Thailand, an area in the pre-elimination phase. Seventy P. vivax isolates, collected between 2017 and 2020, were genotyped for domain II of pvdbp and the 42-kDa region of pvmsp1 using amplicon deep sequencing. Data from Yala province were compared to published data from Tak province, where transmission was higher. Key analyses included nucleotide diversity (π), haplotype diversity (Hd), natural selection, recombination rates, and complexity of infection (COI). Genetic diversity in Yala was relatively low (π = 0.008dbp and 0.014msp1; Hd = 0.774dbp and 0.407msp1) compared to Tak (π = 0.012dbp and 0.027msp1; Hd = 0.849dbp and 0.962msp1). In Yala, polyclonal infections were found in 53.7% of pvdbpII and 47.8% of pvmsp142 isolates, with average COI of 1.6 and 1.7. Both genes were under balancing selection. Distinct genetic differences were found between Yala and Tak in pvmsp142, providing a local genotypic profile useful for tracing parasite origins.