Scholarlink

<p>Camellia-oil trees are economically valuable, oil-rich species within the genus <i>Camellia</i>, family Theaceae. Among these species, <i>C. oleifera</i>, a member of Section <i>Oleifera</i> in the genus, is the most extensively cultivated in China. In this study, we assembled the mitochondrial genomes (mitogenomes) of two Camellia species, namely <i>C. oleifera</i> and <i>C. lanceoleosa</i>. These two species are closely related and belong to the same genus and section, with <i>C. oleifera</i> being hexaploid and <i>C. lanceoleosa</i> being diploid. The mitogenome of <i>C. oleifera</i> is comprised of 1,039,838 base pairs (bp), and <i>C. lanceoleosa</i> is comprised of 934,155&#xa0;bp. Both genomes exhibit a multipartite genome structure, which is supported by our PCR experiments. We conducted codon usage and RNA editing site analysis on these two mitogenomes, which showed highly consistent results. However, analysis of repetitive sequences and mitochondrial plastid sequences (MTPTs) revealed differences between the two mitogenomes. Phylogenetic analysis indicated that these two species clustered together, suggesting a close evolutionary relationship. The collinearity analysis results showed extensive genome rearrangements in the mitogenomes of <i>Camellia</i> species. We successfully assembled the mitogenomes of <i>C. oleifera</i> and <i>C. lanceoleosa</i>, marking a significant advancement in understanding camellia-oil tree mitogenomes. Unlike circular mitogenomes reported before, our research confirms multiple-branched configurations in these two species. This sheds light on mitogenome structural complexities and contributes to our understanding of evolutionary processes. Additionally, these results enrich <i>Camellia</i> genetic resources and expand our knowledge of mitogenome variation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

De novo assembly of the complete mitochondrial genomes of two Camellia-oil tree species reveals their multibranch conformation and evolutionary relationships

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月23日

查看全文
  • Zhun Xiao,
  • Yiyang Gu,
  • Junqin Zhou,
  • Mengqi Lu,
  • Jinfeng Wang,
  • Kaizheng Lu,
  • Yanling Zeng,
  • Xiaofeng Tan

摘要

Camellia-oil trees are economically valuable, oil-rich species within the genus Camellia, family Theaceae. Among these species, C. oleifera, a member of Section Oleifera in the genus, is the most extensively cultivated in China. In this study, we assembled the mitochondrial genomes (mitogenomes) of two Camellia species, namely C. oleifera and C. lanceoleosa. These two species are closely related and belong to the same genus and section, with C. oleifera being hexaploid and C. lanceoleosa being diploid. The mitogenome of C. oleifera is comprised of 1,039,838 base pairs (bp), and C. lanceoleosa is comprised of 934,155 bp. Both genomes exhibit a multipartite genome structure, which is supported by our PCR experiments. We conducted codon usage and RNA editing site analysis on these two mitogenomes, which showed highly consistent results. However, analysis of repetitive sequences and mitochondrial plastid sequences (MTPTs) revealed differences between the two mitogenomes. Phylogenetic analysis indicated that these two species clustered together, suggesting a close evolutionary relationship. The collinearity analysis results showed extensive genome rearrangements in the mitogenomes of Camellia species. We successfully assembled the mitogenomes of C. oleifera and C. lanceoleosa, marking a significant advancement in understanding camellia-oil tree mitogenomes. Unlike circular mitogenomes reported before, our research confirms multiple-branched configurations in these two species. This sheds light on mitogenome structural complexities and contributes to our understanding of evolutionary processes. Additionally, these results enrich Camellia genetic resources and expand our knowledge of mitogenome variation.