Scholarlink

<p>Prior studies examined Acidocin 4356’s antibacterial and antivirulence effects against <i>Pseudomonas aeruginosa</i>, including cell membrane penetration abilities. Building on prior research, an in-vitro co-culture of human cells was established to evaluate the selectivity of Acidocin (ACD) by concurrently cultivating human cells and bacterial pathogens. This study evaluated the antibacterial effectiveness of ACD against <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i>. Laser scanning confocal microscopy (LSCM) and scanning electron microscopy (SEM) revealed significant biofilm dispersion at ACD concentrations as low as 1/2 MIC. The cytotoxicity of ACD was evaluated on two human cell lines, Calu-6 and THP-1, using the MTT assay. The IC<sub>50</sub> values were 114&#xa0;µg/mL and 24&#xa0;µg/mL after a 12-hour treatment duration. In a co-culture model, the IC<sub>50</sub> increased to 118&#xa0;µg/mL, showing greater resilience of THP-1 cells under these settings, mimicking in-vivo conditions. Fluorescent microscopy and flow cytometry analysis confirmed the MTT results, showing ACD’s potent antimicrobial effects and minimal toxicity to human cells, even after 12&#xa0;h of treatment. Transmission electron microscopy (TEM) study revealed that normal Calu-6 cells included papillary outgrowths and microvilli, while infected cells displayed secretory vesicles, indicating an active response to <i>P. aeruginosa</i> infection. The present study thus serves as a critical step toward the development of an innovative therapeutic strategy targeting biofilm-associated infections.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Selective toxicity of a novel antimicrobial peptide Acidocin 4356 against Pseudomonas aeruginosa and Acinetobacter baumannii in human cell-based in vitro infection models

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月19日

查看全文
  • Mahbubeh Talaee,
  • Sima Modiri,
  • Marzieh Rajabi,
  • Fatemeh Saadati,
  • Ali Akbari,
  • Sahar Javadi,
  • Shuaiqi Guo,
  • Hojatollah Vali,
  • Kambiz Akbari Noghabi

摘要

Prior studies examined Acidocin 4356’s antibacterial and antivirulence effects against Pseudomonas aeruginosa, including cell membrane penetration abilities. Building on prior research, an in-vitro co-culture of human cells was established to evaluate the selectivity of Acidocin (ACD) by concurrently cultivating human cells and bacterial pathogens. This study evaluated the antibacterial effectiveness of ACD against Acinetobacter baumannii and Pseudomonas aeruginosa. Laser scanning confocal microscopy (LSCM) and scanning electron microscopy (SEM) revealed significant biofilm dispersion at ACD concentrations as low as 1/2 MIC. The cytotoxicity of ACD was evaluated on two human cell lines, Calu-6 and THP-1, using the MTT assay. The IC50 values were 114 µg/mL and 24 µg/mL after a 12-hour treatment duration. In a co-culture model, the IC50 increased to 118 µg/mL, showing greater resilience of THP-1 cells under these settings, mimicking in-vivo conditions. Fluorescent microscopy and flow cytometry analysis confirmed the MTT results, showing ACD’s potent antimicrobial effects and minimal toxicity to human cells, even after 12 h of treatment. Transmission electron microscopy (TEM) study revealed that normal Calu-6 cells included papillary outgrowths and microvilli, while infected cells displayed secretory vesicles, indicating an active response to P. aeruginosa infection. The present study thus serves as a critical step toward the development of an innovative therapeutic strategy targeting biofilm-associated infections.