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<p>The primary obstacle in detecting bloodstream infections is their often extremely low bacterial concentration. To accurately detect such infections, we developed a quantitative PCR assay, named LowLoad-qPCR, based on the combination of increased input DNA by random priming amplification method and the use of multiple probes sharing a single fluorophore. Here, we detail the key improvements over conventional qPCR and evaluate its performance using blood samples.</p>

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LowLoad-qPCR as a novel clinical strategy for detecting low-load bacteremia

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月3日

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  • Paula Gómez Estévez,
  • José Miguel Cisneros,
  • José Antonio Lepe,
  • Guillermo Martín-Gutiérrez,
  • José Manuel Ortiz de la Rosa

摘要

The primary obstacle in detecting bloodstream infections is their often extremely low bacterial concentration. To accurately detect such infections, we developed a quantitative PCR assay, named LowLoad-qPCR, based on the combination of increased input DNA by random priming amplification method and the use of multiple probes sharing a single fluorophore. Here, we detail the key improvements over conventional qPCR and evaluate its performance using blood samples.