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<p>Catheter-associated urinary tract infections (CAUTI) are complex infections often involving multi-species bacteria. <i>Escherichia coli</i> is frequently an early coloniser. Subsequent colonisation by <i>Pseudomonas aeruginosa</i> and coexistence mechanisms between the two strains within urethral catheters is not yet fully understood. In this study, metabolic adaptations between co-isolated clinical <i>E. coli</i> and <i>P. aeruginosa</i> strains were investigated. It was found that <i>P. aeruginosa</i> outgrew <i>E. coli</i> in artificial urine medium (AUM), whereas <i>E. coli</i> dominated in culture broth such as Iso-sensitest. No evidence of direct antagonism was observed. Metabolite analyses revealed distinct metabolite patterns indicating cross-feeding and metabolic adaptations. In AUM, stress-response metabolites were elevated. Additionally, <i>E. coli</i> appeared to experience Fe-limitation in AUM, while the same was not observed for <i>P. aeruginosa.</i> The results highlight the influence of nutrient conditions on processes within mixed biofilms.</p>

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Metabolic interactions between bacterial co-isolates from catheter-associated urinary tract infections

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月14日

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  • Dmytro Sokol,
  • Olena Rzhepishevska,
  • Iryna Marynova,
  • Tor Monsen,
  • Henrik Antti,
  • Madeleine Ramstedt

摘要

Catheter-associated urinary tract infections (CAUTI) are complex infections often involving multi-species bacteria. Escherichia coli is frequently an early coloniser. Subsequent colonisation by Pseudomonas aeruginosa and coexistence mechanisms between the two strains within urethral catheters is not yet fully understood. In this study, metabolic adaptations between co-isolated clinical E. coli and P. aeruginosa strains were investigated. It was found that P. aeruginosa outgrew E. coli in artificial urine medium (AUM), whereas E. coli dominated in culture broth such as Iso-sensitest. No evidence of direct antagonism was observed. Metabolite analyses revealed distinct metabolite patterns indicating cross-feeding and metabolic adaptations. In AUM, stress-response metabolites were elevated. Additionally, E. coli appeared to experience Fe-limitation in AUM, while the same was not observed for P. aeruginosa. The results highlight the influence of nutrient conditions on processes within mixed biofilms.