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<p>Microbially induced carbonate precipitation (MICP) has proven to be an effective method for soil reinforcement. <i>Sporosarcina pasteurii</i> is widely used due to its high urease activity. However, being an alkaliphilic bacterium, its limitations in acidic soil environments tend to be overlooked. This study isolated a native urease-producing bacterial strain <i>Bacillus aryabhattai</i> with acid tolerance, and comparative analysis of the growth characteristics of <i>B. aryabhattai</i> and <i>S. pasteurii</i>. The grouting and spraying techniques were employed to reinforce granite residual soil by the <i>B. aryabhattai</i> and the <i>S. pasteurii</i>, and the reinforcement mechanisms were systematically investigated. Experimental results indicated that despite exhibiting slightly lower urease activity and growth, the indigenous urease-producing bacterium <i>B. aryabhattai</i> demonstrated superior environmental resilience in terms of both environmental temperature and pH range. The soil samples reinforced by grouting with <i>B. aryabhattai</i> and <i>S. pasteurii</i> exhibited increases in ultrasonic wave velocity, unconfined compressive strength, cohesion, and cumulative disintegration rate to varying degrees compared to the untreated soil samples. Meanwhile, the resistance value of the soil samples reinforced by spraying with <i>B. aryabhattai</i> and <i>S. pasteurii</i> decreased by 84.39% and 79.79%, respectively. Additionally, the calcium carbonate content in the upper section of soil reinforced with <i>B. aryabhattai</i> was comparable to that of <i>S. pasteurii</i>; however, while in the lower section, it exhibited a 36.22% higher precipitation rate than the <i>S. pasteurii</i>-treated soil. Overall, the indigenous strain <i>B. aryabhattai</i> demonstrated remarkable reinforcement effectiveness, attributed to its rapid adaptation to weakly acidic soil conditions and moderate urease activity, which promoted a homogeneous distribution of calcium carbonate. These findings provide significant insights for soil reinforcement applications through MICP.</p>

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Strengthening mechanisms of indigenous bacteria in granite residual soil improvement via microbial induced calcite precipitation

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月16日

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  • Rong Wang,
  • Chao Peng,
  • He Zhao,
  • Haixing Liu,
  • Taibing Wei,
  • Lijuan Wang,
  • Huawei Li

摘要

Microbially induced carbonate precipitation (MICP) has proven to be an effective method for soil reinforcement. Sporosarcina pasteurii is widely used due to its high urease activity. However, being an alkaliphilic bacterium, its limitations in acidic soil environments tend to be overlooked. This study isolated a native urease-producing bacterial strain Bacillus aryabhattai with acid tolerance, and comparative analysis of the growth characteristics of B. aryabhattai and S. pasteurii. The grouting and spraying techniques were employed to reinforce granite residual soil by the B. aryabhattai and the S. pasteurii, and the reinforcement mechanisms were systematically investigated. Experimental results indicated that despite exhibiting slightly lower urease activity and growth, the indigenous urease-producing bacterium B. aryabhattai demonstrated superior environmental resilience in terms of both environmental temperature and pH range. The soil samples reinforced by grouting with B. aryabhattai and S. pasteurii exhibited increases in ultrasonic wave velocity, unconfined compressive strength, cohesion, and cumulative disintegration rate to varying degrees compared to the untreated soil samples. Meanwhile, the resistance value of the soil samples reinforced by spraying with B. aryabhattai and S. pasteurii decreased by 84.39% and 79.79%, respectively. Additionally, the calcium carbonate content in the upper section of soil reinforced with B. aryabhattai was comparable to that of S. pasteurii; however, while in the lower section, it exhibited a 36.22% higher precipitation rate than the S. pasteurii-treated soil. Overall, the indigenous strain B. aryabhattai demonstrated remarkable reinforcement effectiveness, attributed to its rapid adaptation to weakly acidic soil conditions and moderate urease activity, which promoted a homogeneous distribution of calcium carbonate. These findings provide significant insights for soil reinforcement applications through MICP.