Scholarlink

<p>The current study investigated the proangiogenic effects of fraction VII of perivitelline fluid (hscPVF-VEGF) obtained from the late-stage embryos of Indian horseshoe crab (Tachypleus gigas; Müller) using human umbilical cord tissue-derived MSCs (hUCMSCs). Angiogenic potential of hscPVF-VEGF was investigated by analyzing transcripts of signature angiogenic markers, key transcription factors and matrix metalloproteases. Molecular docking studies were performed to predict the binding site of hscPVF-VEGF with the VEGF receptor (VEGFR). hscPVF-VEGF significantly upregulated VEGF, vWF, and downregulated sFlt-1. Significant increase in transcriptional levels of HOXA7, HOXB3, HOXB5, CD31, MMP2, and MMP9 further elucidated the molecular mechanism underlying the angiogenic ability of hscPVF-VEGF. Wound healing assay revealed the migratory potential of hscPVF-VEGF. Molecular docking studies predicted that hscPVF-VEGF may modulate hVEGFR activity by binding in a pocket within the extracellular domains (D5, D6, and D7) distal to the VEGF binding site (D2 and D3). This study infers the potential and molecular mechanism of hscPVF-VEGF inducing angiogenic differentiation in hUCMSCs, suggesting clinical application of a recombinant form of hsPVF-VEGF in disorders with dysfunctional angiogenesis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular insights on the proangiogenic effects of VEGF like growth factor derived from horseshoe crab perivitelline fluid

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月16日

查看全文
  • K. G. Aghila Rani,
  • Hrishikesh Pandit,
  • Rambhadur Subedi,
  • Sayli Otiv-Pandit,
  • Anil Bankati Jogdand,
  • Hajra Gupta,
  • Prayagraj Fandilolu,
  • Sushama Rokade,
  • Susan Idicula-Thomas,
  • Anil Chatterji,
  • Taruna Madan

摘要

The current study investigated the proangiogenic effects of fraction VII of perivitelline fluid (hscPVF-VEGF) obtained from the late-stage embryos of Indian horseshoe crab (Tachypleus gigas; Müller) using human umbilical cord tissue-derived MSCs (hUCMSCs). Angiogenic potential of hscPVF-VEGF was investigated by analyzing transcripts of signature angiogenic markers, key transcription factors and matrix metalloproteases. Molecular docking studies were performed to predict the binding site of hscPVF-VEGF with the VEGF receptor (VEGFR). hscPVF-VEGF significantly upregulated VEGF, vWF, and downregulated sFlt-1. Significant increase in transcriptional levels of HOXA7, HOXB3, HOXB5, CD31, MMP2, and MMP9 further elucidated the molecular mechanism underlying the angiogenic ability of hscPVF-VEGF. Wound healing assay revealed the migratory potential of hscPVF-VEGF. Molecular docking studies predicted that hscPVF-VEGF may modulate hVEGFR activity by binding in a pocket within the extracellular domains (D5, D6, and D7) distal to the VEGF binding site (D2 and D3). This study infers the potential and molecular mechanism of hscPVF-VEGF inducing angiogenic differentiation in hUCMSCs, suggesting clinical application of a recombinant form of hsPVF-VEGF in disorders with dysfunctional angiogenesis.