Scholarlink

<p>Tuberculosis (TB) remains endemic in Oman, with incidence exceeding 5 per 10⁵ population since 2016 and a rising proportion of cases among expatriates (&gt; 40% of the population). This study investigated the spatiotemporal diversity and structure of <i>Mycobacterium tuberculosis</i> (MTB) in Oman between 2008 and 2022. A total of 735 MTB isolates from nationals and expatriates across provinces were genotyped by spoligotyping and 17-locus MIRU-VNTR; 96 underwent whole-genome sequencing (WGS). Genetic diversity (expected heterozygosity, <i>h</i>) and differentiation (<i>F</i>ST) were estimated, and phylogenetic analyses performed. Four major lineages and seven sub-lineages were identified, with similar distributions between nationals and expatriates but temporal variation in spoligotype clades. MIRU-VNTR showed high allelic diversity, with most dominant alleles (76.5%) stable over time, though 32.5% fluctuated. <i>F</i><sub>ST</sub> values (&lt; 0.05) indicated minimal temporal or local differentiation, except for slightly higher values between Dhofar and other regions. WGS revealed close genetic relatedness between Omani and expatriate isolates and strains from expatriates’ countries of origin, suggesting recent cross-border transmission. MTB in Oman exhibits high genetic diversity with a stable temporal structure. Shared lineages between nationals and expatriates underscore the role of migration, supporting the need for integrated genomic surveillance.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Temporal and regional dynamics of Mycobacterium tuberculosis in Oman

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月17日

查看全文
  • Reham Ahmed Hashim,
  • Abeer Al Zubaidi,
  • Fatma Al Ghafri,
  • Amina Al-Jardani,
  • Saleh Al-Azri,
  • Fatma Al Yagoubi,
  • Abdullah A. Balkhair,
  • Arwa Al Kindi,
  • Laila Al Balushi,
  • Samiya Al Zadjali,
  • Salama Al-Hamidhi,
  • Amal A.H. Gadalla,
  • Ahmed Babiker,
  • Lucía Pérez-Pardal,
  • Albano Beja-Pereira,
  • Hamza A Babiker

摘要

Tuberculosis (TB) remains endemic in Oman, with incidence exceeding 5 per 10⁵ population since 2016 and a rising proportion of cases among expatriates (> 40% of the population). This study investigated the spatiotemporal diversity and structure of Mycobacterium tuberculosis (MTB) in Oman between 2008 and 2022. A total of 735 MTB isolates from nationals and expatriates across provinces were genotyped by spoligotyping and 17-locus MIRU-VNTR; 96 underwent whole-genome sequencing (WGS). Genetic diversity (expected heterozygosity, h) and differentiation (FST) were estimated, and phylogenetic analyses performed. Four major lineages and seven sub-lineages were identified, with similar distributions between nationals and expatriates but temporal variation in spoligotype clades. MIRU-VNTR showed high allelic diversity, with most dominant alleles (76.5%) stable over time, though 32.5% fluctuated. FST values (< 0.05) indicated minimal temporal or local differentiation, except for slightly higher values between Dhofar and other regions. WGS revealed close genetic relatedness between Omani and expatriate isolates and strains from expatriates’ countries of origin, suggesting recent cross-border transmission. MTB in Oman exhibits high genetic diversity with a stable temporal structure. Shared lineages between nationals and expatriates underscore the role of migration, supporting the need for integrated genomic surveillance.