Scholarlink

<p>Lung adenocarcinoma (LUAD) is the most prevalent subtype of lung cancer (LC), and the stimulator of interferon genes (STING) is critical in inhibiting its progression. This study investigates the prognostic significance and molecular mechanisms of STING-related genes (STING-RGs) in LUAD. Differential expression analysis, weighted gene co-expression network analysis, as well as Cox regression (CR) identified GAB3 and IL16 as key prognostic genes. A LASSO-based risk model categorized LUAD patients into high-risk group (HRG) and low-risk group (LRG). Patients stratified into the high-risk group (HRG) displayed reduced GAB3 and IL16 expression accompanied by significantly worse survival outcomes. The nomogram combining risk score (RS) with clinical parameters provided robust prognostic discrimination. Comprehensive functional enrichment, immune landscape, and mutational analyses further revealed that the HRG exhibited marked immune-evasive characteristics, while the two risk strata showed differential susceptibilities to multiple chemotherapeutic and targeted therapies. Mutation analysis showed that patients in the Low-TMB + High-risk group had the worst survival outcomes. Western blotting analysis confirmed that GAB3 was downregulated in LUAD tissues. In vitro experiments demonstrated that GAB3 overexpression inhibited cancer cell proliferation and migration, while siRNA-mediated knockdown of GAB3 promoted these processes, suggesting its role as a tumor suppressor gene. In conclusion, GAB3 and IL16 are key prognostic markers, providing insights into STING-related immunotherapy strategies for LUAD.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Prognostic value and biological role of STING-related genes GAB3 and IL16 in lung adenocarcinoma: implications for immune evasion and treatment

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月8日

查看全文
  • Ting Ji,
  • XiaoYan Yang,
  • YongJie Chen,
  • TingTing Zhao,
  • YuXin Xiang,
  • Juan Chen,
  • Kai Yang

摘要

Lung adenocarcinoma (LUAD) is the most prevalent subtype of lung cancer (LC), and the stimulator of interferon genes (STING) is critical in inhibiting its progression. This study investigates the prognostic significance and molecular mechanisms of STING-related genes (STING-RGs) in LUAD. Differential expression analysis, weighted gene co-expression network analysis, as well as Cox regression (CR) identified GAB3 and IL16 as key prognostic genes. A LASSO-based risk model categorized LUAD patients into high-risk group (HRG) and low-risk group (LRG). Patients stratified into the high-risk group (HRG) displayed reduced GAB3 and IL16 expression accompanied by significantly worse survival outcomes. The nomogram combining risk score (RS) with clinical parameters provided robust prognostic discrimination. Comprehensive functional enrichment, immune landscape, and mutational analyses further revealed that the HRG exhibited marked immune-evasive characteristics, while the two risk strata showed differential susceptibilities to multiple chemotherapeutic and targeted therapies. Mutation analysis showed that patients in the Low-TMB + High-risk group had the worst survival outcomes. Western blotting analysis confirmed that GAB3 was downregulated in LUAD tissues. In vitro experiments demonstrated that GAB3 overexpression inhibited cancer cell proliferation and migration, while siRNA-mediated knockdown of GAB3 promoted these processes, suggesting its role as a tumor suppressor gene. In conclusion, GAB3 and IL16 are key prognostic markers, providing insights into STING-related immunotherapy strategies for LUAD.