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<p>The sperm quality, freezability, and fertility of crossbred bulls exhibit significant unpredictability, which consequently impacts the breeding program. The present study documents the supplementation of humanin, a mitochondria-derived peptide, on crossbred bull’s sperm quality, freezability, antioxidant status and in-vitro fertility. For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500&#xa0;million). The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group IV supplemented with 7.5 µM. Semen quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, ejaculate freezability rate, antioxidant status and in-vitro fertility assays were done. At the pre-freeze stage, there was no significant effect on the percentage of individual progressive motility in any group. Supplementation with 5 µM humanin resulted in an 87.5% ejaculate recovery rate after cryopreservation. At the post-thaw stage, sperm quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, antioxidant status and in-vitro fertility were significantly improved in Group III (5 µM humanin supplementation). In conclusion, our results demonstrate that humanin supplementation in crossbred bull semen enhances semen freezability and post-thaw ejaculate recovery rate.</p>

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Mitochondrial derived peptide humanin improved semen quality, semen freezability, antioxidant status and in-vitro fertility in crossbred bull

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月3日

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  • Amare Eshetu Gemeda,
  • Mahak Singh,
  • Rahul Katiyar,
  • Abhishek Kumar,
  • Megha Pande,
  • M. K. Patra,
  • M. Karikalan,
  • N. Srivastava,
  • Vikash Chandra,
  • S. K. Singh,
  • S. K. Ghosh

摘要

The sperm quality, freezability, and fertility of crossbred bulls exhibit significant unpredictability, which consequently impacts the breeding program. The present study documents the supplementation of humanin, a mitochondria-derived peptide, on crossbred bull’s sperm quality, freezability, antioxidant status and in-vitro fertility. For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million). The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group IV supplemented with 7.5 µM. Semen quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, ejaculate freezability rate, antioxidant status and in-vitro fertility assays were done. At the pre-freeze stage, there was no significant effect on the percentage of individual progressive motility in any group. Supplementation with 5 µM humanin resulted in an 87.5% ejaculate recovery rate after cryopreservation. At the post-thaw stage, sperm quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, antioxidant status and in-vitro fertility were significantly improved in Group III (5 µM humanin supplementation). In conclusion, our results demonstrate that humanin supplementation in crossbred bull semen enhances semen freezability and post-thaw ejaculate recovery rate.