Scholarlink

<p>It is well-documented that tissue engineering (TE) has been established as a powerful alternative for treatment of damaged and failed tissues/organs. Therefore, a novel bionanocomposite hydrogel was synthesized based on hydroxyethyl cellulose (HEC), poly(vinyl alcohol) (PVA), and montmorillonite (MMT) clay as a scaffold for bone TE application. For this, HEC and PVA were functionalized with maleic anhydride (MA) to afford HEC-MA and PVA-MA macromonomers. Clay was modified by a methacrylate-end capped silane coupling agent, and then a mixture of HEC-MA, PVA-MA, modified MMT, and <i>N</i>,<i>N´</i>-dimethylaminoethyl methacrylate (DMAEMA) monomer was copolymerized in the presence of a crosslinker <i>via</i> a free radical copolymerization approach to afford a bionanocomposite hydrogel (HEC/PVA-<i>cl</i>-PDMAEMA/MMT). The developed scaffold was loaded with ciprofloxacin (Cip) as an antibiotic drug, and its antibacterial property was investigated by agar well-diffusion method against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. The scaffold showed proper drug loading capacity (4.22%), and pH-responsiveness drug release profile. Hemocompatibility assay exhibited that the scaffold had a hemolysis value less than 5% even at high concentration, which is slightly hemolytic as international standards. As proliferation experiment results by MTT-assay, the scaffold improved attachment, growth and proliferation of human osteoblast-like cells than control group, which qualified it for bone TE.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Antibacterial bionanocomposite hydrogel based on hydroxyethyl cellulose, poly(vinyl alcohol), and montmorillonite clay as a scaffold for bone tissue engineering application

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月2日

查看全文
  • Soheila Zare,
  • Morteza Eskandani,
  • Rana Jahanban Esfahlan,
  • Mehdi Jaymand

摘要

It is well-documented that tissue engineering (TE) has been established as a powerful alternative for treatment of damaged and failed tissues/organs. Therefore, a novel bionanocomposite hydrogel was synthesized based on hydroxyethyl cellulose (HEC), poly(vinyl alcohol) (PVA), and montmorillonite (MMT) clay as a scaffold for bone TE application. For this, HEC and PVA were functionalized with maleic anhydride (MA) to afford HEC-MA and PVA-MA macromonomers. Clay was modified by a methacrylate-end capped silane coupling agent, and then a mixture of HEC-MA, PVA-MA, modified MMT, and N,N´-dimethylaminoethyl methacrylate (DMAEMA) monomer was copolymerized in the presence of a crosslinker via a free radical copolymerization approach to afford a bionanocomposite hydrogel (HEC/PVA-cl-PDMAEMA/MMT). The developed scaffold was loaded with ciprofloxacin (Cip) as an antibiotic drug, and its antibacterial property was investigated by agar well-diffusion method against Staphylococcus aureus and Escherichia coli. The scaffold showed proper drug loading capacity (4.22%), and pH-responsiveness drug release profile. Hemocompatibility assay exhibited that the scaffold had a hemolysis value less than 5% even at high concentration, which is slightly hemolytic as international standards. As proliferation experiment results by MTT-assay, the scaffold improved attachment, growth and proliferation of human osteoblast-like cells than control group, which qualified it for bone TE.