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<p>Intracranial hemorrhage (ICH) remains a challenging disease worldwide with high mortality, morbidity, and disability. The combination of brain-derived neurotrophic factor (BDNF) therapy and hydrogel implantation is a potential novel treatment for promoting neuroprotection, neurogenesis, and neurological recovery. Hydrogel may serve as a vector for gene delivery. This study examined the therapeutic efficacy of BDNF plasmid delivered with a chitosan-based self-healing hydrogel in treating ICH rats. Direct injection of BDNF plasmid-containing hydrogel into bled brain tissue caused an approximately 1.8-fold increase in BDNF expression and behavioral improvements of at least 80%. Furthermore, medical imaging revealed that untreated rats experienced approximately 6% brain atrophy, while BDNF plasmid-treated rats showed reduced atrophy of 2%. Postmortem histological examination revealed that compared to untreated rats, rats treated with BDNF had a five fold increase in the number of neuroblasts, whereas the number of macrophages decreased by half. Conclusively, injection of self-healing hydrogel with BDNF plasmid may be a novel gene therapy approach for post-ICH treatment.</p>

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BDNF plasmid hydrogel promotes neuroprotection and neurogenesis in rats with intracerebral hemorrhage

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月23日

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  • Abel Po-Hao Huang,
  • Yi-Hua Hsu,
  • Tzu-Hua Chen,
  • William Wei-Lin Pan,
  • Kelly Hsin-Ju Chen,
  • Po-An Tai,
  • Joy Wang,
  • Dar-Ming Lai,
  • Shan-hui Hsu

摘要

Intracranial hemorrhage (ICH) remains a challenging disease worldwide with high mortality, morbidity, and disability. The combination of brain-derived neurotrophic factor (BDNF) therapy and hydrogel implantation is a potential novel treatment for promoting neuroprotection, neurogenesis, and neurological recovery. Hydrogel may serve as a vector for gene delivery. This study examined the therapeutic efficacy of BDNF plasmid delivered with a chitosan-based self-healing hydrogel in treating ICH rats. Direct injection of BDNF plasmid-containing hydrogel into bled brain tissue caused an approximately 1.8-fold increase in BDNF expression and behavioral improvements of at least 80%. Furthermore, medical imaging revealed that untreated rats experienced approximately 6% brain atrophy, while BDNF plasmid-treated rats showed reduced atrophy of 2%. Postmortem histological examination revealed that compared to untreated rats, rats treated with BDNF had a five fold increase in the number of neuroblasts, whereas the number of macrophages decreased by half. Conclusively, injection of self-healing hydrogel with BDNF plasmid may be a novel gene therapy approach for post-ICH treatment.