Scholarlink

<p>Implant-associated biofilm infections, particularly those caused by <i>Staphylococcus aureus</i> and <i>Enterococcus faecalis</i>, present significant challenges in clinical settings, often necessitating surgical removal. This study investigates the potential of the invertebrate model <i>Galleria mellonella</i> for evaluating biofilm formation of <i>S. aureus</i> and <i>E. faecalis</i> clinical isolates, the leading causes of implant-associated infections. Utilizing expanded polytetrafluoroethylene (ePTFE) sutures as a surrogate for cardiac implants, we employed two biofilm formation methodologies reflecting the two main routes of implant infections: <i>in vivo</i> biofilm formation within larvae mimicking pathogen spread and pre-formed biofilm transplantation. Scanning electron microscopy revealed complex biofilm structures for the biofilm formed inside of the larvae on implant, closely mimicking clinical implant biofilm. Antibiotic combination of vancomycin and rifampicin demonstrated mean 5 log<sub>10</sub> reductions in bacterial CFU count per larva and 50% improved survival, proving highly effective. The study highlights the <i>G. mellonella</i> model’s potential for preclinical biofilm research, offering a cost-effective and ethical alternative to vertebrate models while providing valuable insights into biofilm-related infections and their treatment.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Implant-associated biofilms of Staphylococcus aureus and Enterococcus faecalis clinical isolates on expanded polytetrafluoroethylene suture in Galleria mellonella model

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月12日

查看全文
  • Kamran Ahmad Mirza,
  • Sandor Nietzsche,
  • Tinatini Tchatchiashvili,
  • Oliwia Makarewicz,
  • Mathias W. Pletz,
  • Lara Thieme

摘要

Implant-associated biofilm infections, particularly those caused by Staphylococcus aureus and Enterococcus faecalis, present significant challenges in clinical settings, often necessitating surgical removal. This study investigates the potential of the invertebrate model Galleria mellonella for evaluating biofilm formation of S. aureus and E. faecalis clinical isolates, the leading causes of implant-associated infections. Utilizing expanded polytetrafluoroethylene (ePTFE) sutures as a surrogate for cardiac implants, we employed two biofilm formation methodologies reflecting the two main routes of implant infections: in vivo biofilm formation within larvae mimicking pathogen spread and pre-formed biofilm transplantation. Scanning electron microscopy revealed complex biofilm structures for the biofilm formed inside of the larvae on implant, closely mimicking clinical implant biofilm. Antibiotic combination of vancomycin and rifampicin demonstrated mean 5 log10 reductions in bacterial CFU count per larva and 50% improved survival, proving highly effective. The study highlights the G. mellonella model’s potential for preclinical biofilm research, offering a cost-effective and ethical alternative to vertebrate models while providing valuable insights into biofilm-related infections and their treatment.