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<p>Phenolic acids in wheat bran, particularly ferulic acid (FA), exhibit antimicrobial properties, but most FA is bound to cell wall polysaccharides via ester/ether linkages, severely limiting its bioavailability and extraction. Distinct from prior studies relying on commercial enzymes or single-component substrates, this study enhanced FA release through <i>Aspergillus niger</i> (<i>A. niger</i>) fermentation (no exogenous enzymes), utilizing a wheat bran-vinegar residue (9:1, w/w) substrate. Fermentation and destarched wheat bran (DSWB) enzymatic hydrolysis were optimized via Box-Behnken design and response surface methodology (BBD-RSM), yielding high enzyme activities: feruloyl esterase (16.63 ± 0.32 U/g), xylanase (3098.21 ± 47.27 U/g), and cellulase (21.12 ± 0.14 U/g). Hydrolysis released 5.41 ± 0.03&#xa0;mg/g total FA (TFA) (including derivatives), which exerted significant antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>, with MIC/MBC of 0.6/2.4&#xa0;mg/mL (<i>E. coli</i>) and 0.3/1.2&#xa0;mg/mL (<i>S. aureus</i>). This study offers valuable data for wheat bran-derived antimicrobials and enzyme-treated bran as feed antibiotic alternatives.</p>

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Optimization of Aspergillus Niger fermentation for enzyme production and enzymatic hydrolysis of wheat bran for total ferulic acid via RSM

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月27日

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  • Liujun Chen,
  • Jing Zhao,
  • Pengpeng Li,
  • Tianyou Kong,
  • Jia Gao,
  • Xiangxin Han,
  • Shuming Chen,
  • Xiaomin Guo

摘要

Phenolic acids in wheat bran, particularly ferulic acid (FA), exhibit antimicrobial properties, but most FA is bound to cell wall polysaccharides via ester/ether linkages, severely limiting its bioavailability and extraction. Distinct from prior studies relying on commercial enzymes or single-component substrates, this study enhanced FA release through Aspergillus niger (A. niger) fermentation (no exogenous enzymes), utilizing a wheat bran-vinegar residue (9:1, w/w) substrate. Fermentation and destarched wheat bran (DSWB) enzymatic hydrolysis were optimized via Box-Behnken design and response surface methodology (BBD-RSM), yielding high enzyme activities: feruloyl esterase (16.63 ± 0.32 U/g), xylanase (3098.21 ± 47.27 U/g), and cellulase (21.12 ± 0.14 U/g). Hydrolysis released 5.41 ± 0.03 mg/g total FA (TFA) (including derivatives), which exerted significant antibacterial activity against Escherichia coli and Staphylococcus aureus, with MIC/MBC of 0.6/2.4 mg/mL (E. coli) and 0.3/1.2 mg/mL (S. aureus). This study offers valuable data for wheat bran-derived antimicrobials and enzyme-treated bran as feed antibiotic alternatives.