Scholarlink

<p>Human dental pulp stem cells (hDPSCs) senescence impairs their proliferation and osteogenic differentiation, critical for dental stem cell therapy. This study evaluated the effects of Resveratrol on the senescence of hDPSCs to explore new therapeutic strategies. Metabolomic analysis identified age-related metabolic differences in dental pulp tissues, with enriched pathways linked to Resveratrol. In vitro, Resveratrol improved proliferation, delayed senescence, promoted osteogenic differentiation, and enhanced mitochondrial autophagy, function, and biogenesis in senescent hDPSCs, reducing mitochondrial damage and oxidative stress. Mechanistically, silencing PINK1 or PGC-1α reversed Resveratrol-mediated promotion of proliferation, osteogenesis, and senescence suppression. Blocking SIRT1 abrogated its effects on mitochondrial quality control. These findings highlight Resveratrol’s potential to mitigate hDPSCs senescence via SIRT1-dependent mitochondrial regulation, offering insights for age-related dental regenerative therapies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Resveratrol delays senescence of human dental pulp stem cells via activating the SIRT1-mitochondrial autophagy

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月21日

查看全文
  • Zelu Li,
  • Xiaoyang Chu,
  • Jiahao Guo,
  • Yulan Xiang,
  • Yingyi Luan,
  • Hongcheng Xing,
  • Xixi Lv,
  • Yanhong Zhou,
  • Kai Yang,
  • Yang Zeng,
  • Dongliang Zhang

摘要

Human dental pulp stem cells (hDPSCs) senescence impairs their proliferation and osteogenic differentiation, critical for dental stem cell therapy. This study evaluated the effects of Resveratrol on the senescence of hDPSCs to explore new therapeutic strategies. Metabolomic analysis identified age-related metabolic differences in dental pulp tissues, with enriched pathways linked to Resveratrol. In vitro, Resveratrol improved proliferation, delayed senescence, promoted osteogenic differentiation, and enhanced mitochondrial autophagy, function, and biogenesis in senescent hDPSCs, reducing mitochondrial damage and oxidative stress. Mechanistically, silencing PINK1 or PGC-1α reversed Resveratrol-mediated promotion of proliferation, osteogenesis, and senescence suppression. Blocking SIRT1 abrogated its effects on mitochondrial quality control. These findings highlight Resveratrol’s potential to mitigate hDPSCs senescence via SIRT1-dependent mitochondrial regulation, offering insights for age-related dental regenerative therapies.