Scholarlink

<p>Vascular composite allotransplantation (VCA) is a promising reconstructive surgical option for severe tissue loss. Autologous reconstructive methods are limited by donor tissue availability and donor site morbidity. Long-term immunosuppression regimens have severe side effects that limit the clinical implications of VCA. Tissue engineered composite grafts using decellularization and recellularization represent a potential solution to this. We present ex vivo recellularization of decellularized rat hindlimbs using human umbilical vein endothelial cells (HUVECs) and L6 rat myoblasts. HUVECs and L6 rat myoblasts were seeded in the vascular and muscle compartments, respectively and cultured up to 7 days ex vivo. We report cell engraftment in femoral vessels, small vessels located distally in muscle, and cell clusters in muscle, with phenotypic characterization in both tissue types. Formation of a neo-endothelium and nascent myotube formation was observed. Cell survival in both vessels and muscle was also demonstrated. These findings provide a foundational basis for a proof-of-concept small animal model for recellularization of composite tissues for VCA.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Ex vivo perfusion recellularization of acellular rat hindlimbs for vascular composite allotransplantation

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月21日

查看全文
  • Aisha Adil,
  • Felor Biniazan,
  • Valentina Shamoun,
  • Golnaz Karoubi,
  • Siba Haykal

摘要

Vascular composite allotransplantation (VCA) is a promising reconstructive surgical option for severe tissue loss. Autologous reconstructive methods are limited by donor tissue availability and donor site morbidity. Long-term immunosuppression regimens have severe side effects that limit the clinical implications of VCA. Tissue engineered composite grafts using decellularization and recellularization represent a potential solution to this. We present ex vivo recellularization of decellularized rat hindlimbs using human umbilical vein endothelial cells (HUVECs) and L6 rat myoblasts. HUVECs and L6 rat myoblasts were seeded in the vascular and muscle compartments, respectively and cultured up to 7 days ex vivo. We report cell engraftment in femoral vessels, small vessels located distally in muscle, and cell clusters in muscle, with phenotypic characterization in both tissue types. Formation of a neo-endothelium and nascent myotube formation was observed. Cell survival in both vessels and muscle was also demonstrated. These findings provide a foundational basis for a proof-of-concept small animal model for recellularization of composite tissues for VCA.