Scholarlink

<p>Toxin-Antitoxin (TA) systems are highly conserved genetic elements in the <i>Mycobacterium tuberculosis</i> complex, playing a critical role in bacterial survival, stress response, and persistence. Small variations in sequence composition can lead to species-specific functional differences, potentially influencing host adaptation, antibiotic tolerance, and virulence strategies. In this study, a comparative analysis of TA systems in <i>M. tuberculosis</i> and <i>M. bovis</i> was conducted using BLAST NCBI, and SnapGene 5.3.1. A main mutation was identified at nucleotide 719 of the <i>vapC3</i> gene in <i>M. bovis</i> isolates. While <i>vapC3</i> in <i>M. tuberculosis</i> consists of 137 amino acids, a nucleotide deletion in <i>M. bovis</i> resulted in a truncated protein of only 109 amino acids in the primary sequence, significantly altering its structure. These findings suggest potential differences in the translation and function of <i>vapC3</i> between the two species. Molecular docking simulations were conducted using HADDOCK 2.4 to assess the functional implications of <i>vapC3</i> mutations. The analysis revealed that VapBC3 in <i>M. bovis</i> exhibits a more stable interaction than in <i>M. tuberculosis</i>, which could be crucial in understanding species-specific toxin-antitoxin system functionality. Future studies should focus on the impact of this mutation on VapC3-mediated stress response, experimental validation and therapeutic applications targeting VapBC3-mediated bacterial survival pathways.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Comparative in silico analysis of vapBC3 toxin-antitoxin system in Mycobacterium Bovis and Mycobacterium tuberculosis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月19日

查看全文
  • Maryam Shafipour,
  • Abdolmajid Mohammadzadeh,
  • Gholamhossein Farrokhpour Tabrizi,
  • Pezhman Mahmoodi,
  • Ezzat Allah Ghaemi

摘要

Toxin-Antitoxin (TA) systems are highly conserved genetic elements in the Mycobacterium tuberculosis complex, playing a critical role in bacterial survival, stress response, and persistence. Small variations in sequence composition can lead to species-specific functional differences, potentially influencing host adaptation, antibiotic tolerance, and virulence strategies. In this study, a comparative analysis of TA systems in M. tuberculosis and M. bovis was conducted using BLAST NCBI, and SnapGene 5.3.1. A main mutation was identified at nucleotide 719 of the vapC3 gene in M. bovis isolates. While vapC3 in M. tuberculosis consists of 137 amino acids, a nucleotide deletion in M. bovis resulted in a truncated protein of only 109 amino acids in the primary sequence, significantly altering its structure. These findings suggest potential differences in the translation and function of vapC3 between the two species. Molecular docking simulations were conducted using HADDOCK 2.4 to assess the functional implications of vapC3 mutations. The analysis revealed that VapBC3 in M. bovis exhibits a more stable interaction than in M. tuberculosis, which could be crucial in understanding species-specific toxin-antitoxin system functionality. Future studies should focus on the impact of this mutation on VapC3-mediated stress response, experimental validation and therapeutic applications targeting VapBC3-mediated bacterial survival pathways.