<p>Water inrush from confined aquifers in coal seam floor poses a great threat to the safety production of coal mines, while the aquiclude in coal seam floor is a key factor to avoid water inrush disasters. Therefore, an objective and accurate evaluation on the water-resisting ability of the aquiclude can effectively identify potential risk areas of water inrush and plays an important role in preventing such disasters. In this study, Longfeng coal mine was taken as an example. And based on the availability of data and the representativeness of factors, thickness of effective aquiclude, brittle rock thickness of aquiclude, rock quality designation of aquiclude, consumption of drilling fluid, mudstone ratio of aquiclude and fault fracture zone were selected as evaluation factors for water-resisting ability. Besides, the non-linear mathematical method—entropy weight method was combined to construct a comprehensive evaluation model of the water-resisting ability of aquiclude in coal seam floor. On this basis, the information processing and spatial display functions of GIS technology were used to make a thematic map of influencing factors and the thematic map was compositely superimposed. Finally, the evaluation on the water-resisting ability of aquiclude in coal seam floor in Longfeng coal mine was realized. The multi-source information fusion evaluation method based on GIS and entropy weight can comprehensively and objectively reflect the feature that the water-resisting ability of aquiclude in coal seam floor was controlled by multiple factors and had complex formation mechanisms. Also, the evaluation results were displayed intuitively and visually and can provide a scientific basis for the prevention and control of water inrush disasters from coal seam floor.</p>