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<p>The spectrum of insecticidal activity of the Cry1B.3, Cry1Da_7, and Vip3Cb1 proteins targeting lepidopteran insect pests produced by MON 89151 cotton was characterized with microbially-produced protein-incorporated diet bioassays against selected insect species from four orders and nine families. Cry1B.3 and Cry1Da_7 are <i>Bacillus thuringiensis</i> (<i>Bt</i>) insecticidal proteins, whereas the Vip3Cb1 protein is derived from <i>Paenibacillus</i> spp. in the <i>P. popilliae</i>-containing clade. Notably, Cry1B.3 has the same functional region as the Cry1B.2 protein in MON 94637 soybean, and Cry1Da_7 is identical to the protein expressed in MON 95379 maize; therefore, existing spectrum of activity data for Cry1B.2 and Cry1Da_7 were utilized to inform the characterization of Cry1B.3 and Cry1Da_7 in MON 89151 cotton. Overall, our results demonstrate that Cry1B.3, Cry1Da_7, and Vip3Cb1 exhibit a narrow spectrum of activity limited to the lepidopteran pests tested. Thus, these insecticidal proteins are valuable additions to the cotton trait portfolio to manage lepidopteran pests with a high degree of specificity.</p>

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Characterization of the spectrum of insecticidal activity of Cry1B.3, Cry1Da_7, and Vip3Cb1 proteins produced by lepidopteran-protected MON 89151 cotton

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月17日

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  • Carlos J. Esquivel,
  • Frankie Stubbins,
  • Harit K. Bal,
  • Jianguo Tan,
  • Brent Werner,
  • Steven Michael Reyna,
  • Jennifer Fridley,
  • Geoffrey Mueller,
  • Yu Liu-Gontarek,
  • Bingyao Li,
  • Chen Meng,
  • Christopher R. Brown,
  • Tianbo Xu,
  • Steven L. Levine,
  • Yong Yin,
  • Chitvan Khajuria

摘要

The spectrum of insecticidal activity of the Cry1B.3, Cry1Da_7, and Vip3Cb1 proteins targeting lepidopteran insect pests produced by MON 89151 cotton was characterized with microbially-produced protein-incorporated diet bioassays against selected insect species from four orders and nine families. Cry1B.3 and Cry1Da_7 are Bacillus thuringiensis (Bt) insecticidal proteins, whereas the Vip3Cb1 protein is derived from Paenibacillus spp. in the P. popilliae-containing clade. Notably, Cry1B.3 has the same functional region as the Cry1B.2 protein in MON 94637 soybean, and Cry1Da_7 is identical to the protein expressed in MON 95379 maize; therefore, existing spectrum of activity data for Cry1B.2 and Cry1Da_7 were utilized to inform the characterization of Cry1B.3 and Cry1Da_7 in MON 89151 cotton. Overall, our results demonstrate that Cry1B.3, Cry1Da_7, and Vip3Cb1 exhibit a narrow spectrum of activity limited to the lepidopteran pests tested. Thus, these insecticidal proteins are valuable additions to the cotton trait portfolio to manage lepidopteran pests with a high degree of specificity.