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<p>In eukaryotic cells, histone acetylation and chromatin states affect gene expression programs in broad spectrum of cellular function. Comprehending the genomic signature of retinoid X receptor (RXR) will provide crucial insight into the molecular signaling pathways underlying stem cell fate transition. Here, we employ an integrative ChIP-seq approach to investigate the interplay of RXR with MyoD and CTCF in proliferating myoblasts. Our findings suggest an active enhancer distribution of RXR along with MyoD, marked by H3K18 and H3K27 acetylation, potentially mediated by histone acetyltransferase p300. On the other hand, RXR together with CTCF were mainly distributed to active promoters, marked by H3K9 acetylation. CTCF in proximity to RXR was also associated to nuclear receptor regulator NCOA1 and N-COR, suggesting a potential role in chromatin organization. Taken together, our analyses indicate a crucial interplay of MyoD and CTCF with RXR in the regulation of stem cell function.</p>

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Cistromic insight into the association of retinoid X receptor with MyoD and CTCF in proliferating myoblasts

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月6日

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  • Jean-Thomas Clément,
  • Qiao Li

摘要

In eukaryotic cells, histone acetylation and chromatin states affect gene expression programs in broad spectrum of cellular function. Comprehending the genomic signature of retinoid X receptor (RXR) will provide crucial insight into the molecular signaling pathways underlying stem cell fate transition. Here, we employ an integrative ChIP-seq approach to investigate the interplay of RXR with MyoD and CTCF in proliferating myoblasts. Our findings suggest an active enhancer distribution of RXR along with MyoD, marked by H3K18 and H3K27 acetylation, potentially mediated by histone acetyltransferase p300. On the other hand, RXR together with CTCF were mainly distributed to active promoters, marked by H3K9 acetylation. CTCF in proximity to RXR was also associated to nuclear receptor regulator NCOA1 and N-COR, suggesting a potential role in chromatin organization. Taken together, our analyses indicate a crucial interplay of MyoD and CTCF with RXR in the regulation of stem cell function.