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<p>In the present study, an ultra-sensitive, very simple, and specific fluorescence resonance energy transfer (FRET)-based aptasensor was developed to detect C-reactive protein (CRP). CRP is a critical clinical biomarker and a sensitive indicator of inflammation, tissue damage, and infectious illnesses. Our turn off-on aptasensor system utilizes 6-carboxyfluorescein (5-FAM) dye-labeled aptamer assembled on graphene oxide (GO). The noncovalent assembly between the aptamer and GO initially quenches the fluorescence of the dye through FRET, creating a “turn-off” state. Upon addition of CRP protein, the aptamer binds to CRP, causing the dye to separate from GO, thereby recovering the fluorescence signal and creating a “turn-on” state. Under optimal conditions, the aptasensor exhibits two linear ranges (33–82&#xa0;fg/ml and 114–207&#xa0;fg/ml) and an exceptional limit of detection (LOD) of 2.27&#xa0;fg/ml. This ultra-sensitive aptasensor demonstrates excellent selectivity, as potential interference from other substances was negligible. Compared to other methods, our proposed turn off-on aptasensor-assembled GO offers a more extensive detection range and significantly higher sensitivity. Additionally, it yields satisfactory results by applying 1&#xa0;µl of real positive and negative serum samples, showcasing its potential for practical applications in clinical diagnostics.</p>

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Very simple and ultra-sensitive fluorescence resonance energy transfer aptasensor for turn-on C-reactive protein detection in biological matrices

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月30日

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  • Elham Fathi ganje lou,
  • Ali Hossein Rezayan,
  • Marjan Malekmohamadi,
  • Vahid Abbasi,
  • Fatemeh Molaabasi

摘要

In the present study, an ultra-sensitive, very simple, and specific fluorescence resonance energy transfer (FRET)-based aptasensor was developed to detect C-reactive protein (CRP). CRP is a critical clinical biomarker and a sensitive indicator of inflammation, tissue damage, and infectious illnesses. Our turn off-on aptasensor system utilizes 6-carboxyfluorescein (5-FAM) dye-labeled aptamer assembled on graphene oxide (GO). The noncovalent assembly between the aptamer and GO initially quenches the fluorescence of the dye through FRET, creating a “turn-off” state. Upon addition of CRP protein, the aptamer binds to CRP, causing the dye to separate from GO, thereby recovering the fluorescence signal and creating a “turn-on” state. Under optimal conditions, the aptasensor exhibits two linear ranges (33–82 fg/ml and 114–207 fg/ml) and an exceptional limit of detection (LOD) of 2.27 fg/ml. This ultra-sensitive aptasensor demonstrates excellent selectivity, as potential interference from other substances was negligible. Compared to other methods, our proposed turn off-on aptasensor-assembled GO offers a more extensive detection range and significantly higher sensitivity. Additionally, it yields satisfactory results by applying 1 µl of real positive and negative serum samples, showcasing its potential for practical applications in clinical diagnostics.