<p>Electron microscopy (EM) of rhinovirus A2 (RV-A2) incubated in Na⁺ phosphate buffer (pH 7.6) for 12&#xa0;h at 25&#xa0;°C revealed partial fragmentation, whereas upon incubation in K⁺ phosphate buffer, RV-A2 appeared intact. In buffers adjusted to pH 5.8, these differences became more pronounced; acidic Na⁺ phosphate buffer promoted disintegration of the particles, whereas in acidic K⁺ phosphate buffer, the virus appeared like native. Incubation in the acidic buffers for one hour at 4&#xa0;°C followed by neutralisation resulted in the respective formation of non-infectious A particles (in Na⁺) and a non-infectious novel uncoating intermediate (in K⁺), which we termed ‘E0 particle’. Negative staining EM revealed phosphotungstate penetration into A particles, but not into E0 particles. Cryo-EM image reconstruction of the E0 particle showed clear differences between A and E0 particles; like native virus, E0 contained VP4 and a pocket factor. Native RV-A2 RNA cores, obtained by gentle proteinase-K digestion in K⁺ and Na⁺ phosphate buffer, respectively, differed in accessibility of dsRNA regions, detected by PaSTRy. Variance in RNA compactness observed in K⁺ versus Na⁺ phosphate buffer was confirmed by rotary shadowing EM; in K⁺ phosphate buffer, the RNA remained condensed while, in Na⁺ phosphate buffer, distinct unfolding stages were apparent.</p>