Scholarlink

<p><i>V. parahaemolyticus</i> poses a remarkable public health threat, accounting for approximately 25% of global seafood-related infections in human consumers and resulting in severe infections and substantial economic losses in the aquaculture. To explore the prevalence, antibiogram, virulence and resistance genes, the multidrug resistance profiles, and the pathogenicity of <i>V. parahaemolyticus</i> recovered from shrimp, 200 <i>Litopenaeus vannamei</i> (clinically healthy: <i>n</i> = 100 and diseased: <i>n</i> = 100) were gathered from commercial shrimp farms in Ismailia, Egypt. Accordingly, clinical and postmortem findings and bacteriological examinations were performed. All the recovered isolates were positive for the <i>gro</i>EL and <i>Ap3</i> genes, indicating that all the retrieved isolates were AHPND-causing strains. The prevalence of <i>V. parahaemolyticus</i> in the examined shrimp was 11% (22/200), where the hepatopancreas was the prominent infected organ. Using PCR, the prevalence of the <i>tox</i>R, <i>tlh</i>, <i>tdh</i>, and <i>trh</i> virulence genes was 100%, 98%, 80%, and 28%, respectively. Moreover, 42% of the obtained <i>V. parahaemolyticus</i> strains were MDR to seven antimicrobial classes and had the <i>bla</i><sub>TEM</sub>, <i>tet</i>A, <i>bla</i><sub>OXA</sub>, <i>sul</i>1, <i>aad</i>A, and <i>erm</i>B genes. In addition, 16% of the isolated strains were MDR to six classes and had the <i>bla</i><sub>TEM</sub>, <i>tet</i>A, <i>bla</i><sub>OXA</sub>, <i>aad</i>A, and <i>erm</i>B genes. The pathogenicity trial emphasized the positive correlation between the inherited virulence genes of the tested strains and the recorded mortalities. In brief, this investigation highlighted the development of MDR <i>V. parahaemolyticus</i> in shrimp, affirming a public health threat. The evolving MDR <i>V. parahaemolyticus</i> strains usually carry the <i>Ap3</i>, <i>tox</i>R, <i>tlh</i>, and <i>tdh</i> virulence genes, and the <i>erm</i>B, <i>bla</i><sub>TEM</sub>, <i>aad</i>A, <i>bla</i><sub>OXA</sub>, <i>sul</i>1, <i>tet</i>A, and/or <i>tet</i>B antibiotic resistance genes.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

In-depth characterization of virulence traits, pathogenicity, antibiogram, and antibiotic resistance genes of MDR Vibrio parahaemolyticus retrieved from shrimp

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月1日

查看全文
  • Abdelazeem M. Algammal,
  • Saad Alghamdi,
  • Bian K. Almessiry,
  • Ahmed Kabrah,
  • Azhar S. Sindi,
  • Mohammad Y. Alshahrani,
  • Ebtesam Al-Olayan,
  • Fatma M. Youssef,
  • Sara S. Abdelsamea,
  • Geraldine B. Dayrit,
  • Mahmoud Mabrok

摘要

V. parahaemolyticus poses a remarkable public health threat, accounting for approximately 25% of global seafood-related infections in human consumers and resulting in severe infections and substantial economic losses in the aquaculture. To explore the prevalence, antibiogram, virulence and resistance genes, the multidrug resistance profiles, and the pathogenicity of V. parahaemolyticus recovered from shrimp, 200 Litopenaeus vannamei (clinically healthy: n = 100 and diseased: n = 100) were gathered from commercial shrimp farms in Ismailia, Egypt. Accordingly, clinical and postmortem findings and bacteriological examinations were performed. All the recovered isolates were positive for the groEL and Ap3 genes, indicating that all the retrieved isolates were AHPND-causing strains. The prevalence of V. parahaemolyticus in the examined shrimp was 11% (22/200), where the hepatopancreas was the prominent infected organ. Using PCR, the prevalence of the toxR, tlh, tdh, and trh virulence genes was 100%, 98%, 80%, and 28%, respectively. Moreover, 42% of the obtained V. parahaemolyticus strains were MDR to seven antimicrobial classes and had the blaTEM, tetA, blaOXA, sul1, aadA, and ermB genes. In addition, 16% of the isolated strains were MDR to six classes and had the blaTEM, tetA, blaOXA, aadA, and ermB genes. The pathogenicity trial emphasized the positive correlation between the inherited virulence genes of the tested strains and the recorded mortalities. In brief, this investigation highlighted the development of MDR V. parahaemolyticus in shrimp, affirming a public health threat. The evolving MDR V. parahaemolyticus strains usually carry the Ap3, toxR, tlh, and tdh virulence genes, and the ermB, blaTEM, aadA, blaOXA, sul1, tetA, and/or tetB antibiotic resistance genes.