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<p>The pathogenicity of <i>Klebsiella pneumoniae</i> is continuously on the rise owing to variations in virulence. The <i>polyketide synthase</i> (<i>pks</i>) gene plays a crucial role in the expression of microbial virulence genes. However, <i>Klebsiella pneumoniae</i>, often considered a colonizing bacterium, has been shown to possess significant virulence and antibiotic resistance properties, which might be a significant factor contributing to the persistent difficulty in curing clinical gastrointestinal infections. This study analyzed the <i>pks</i> gene expression in <i>Klebsiella pneumoniae</i> and the associated clinical features in digestive infections over the past year. Gene editing technology and genomic analysis were employed to identify the virulence genes of <i>Klebsiella pneumoniae</i> in digestive tract infections. Experimental results demonstrated that a higher proportion of <i>pks</i>-positive <i>Klebsiella pneumoniae</i> were detected in the digestive tracts of clinical patients, and these strains carried more virulence factors. The <i>clbA</i> gene fragment can be utilized independently as a detection fragment for the <i>pks</i> gene. The <i>pks</i> gene may influence the virulence expression of <i>Klebsiella pneumoniae</i> through different signal pathways associated with other virulent genes. Testing for the <i>pks</i> gene can offer experimental evidence to support clinical treatment decisions.</p>

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High polyketide synthase gene expression boosts pathogenicity of Klebsiella pneumoniae in the digestive infection

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月14日

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  • Chenshuo Luo,
  • Weiwen Huang,
  • Yue Shi,
  • Shanjian Chen,
  • Yulan Lin,
  • Bin Yang

摘要

The pathogenicity of Klebsiella pneumoniae is continuously on the rise owing to variations in virulence. The polyketide synthase (pks) gene plays a crucial role in the expression of microbial virulence genes. However, Klebsiella pneumoniae, often considered a colonizing bacterium, has been shown to possess significant virulence and antibiotic resistance properties, which might be a significant factor contributing to the persistent difficulty in curing clinical gastrointestinal infections. This study analyzed the pks gene expression in Klebsiella pneumoniae and the associated clinical features in digestive infections over the past year. Gene editing technology and genomic analysis were employed to identify the virulence genes of Klebsiella pneumoniae in digestive tract infections. Experimental results demonstrated that a higher proportion of pks-positive Klebsiella pneumoniae were detected in the digestive tracts of clinical patients, and these strains carried more virulence factors. The clbA gene fragment can be utilized independently as a detection fragment for the pks gene. The pks gene may influence the virulence expression of Klebsiella pneumoniae through different signal pathways associated with other virulent genes. Testing for the pks gene can offer experimental evidence to support clinical treatment decisions.