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<p>Odorant receptor-expressing cells have been shown to recognize various odors, which has brought them to the interest of the growing field of cell-based olfactory sensors. However, cell cultures are difficult to use outside a laboratory because of their continuous need for controlled conditions. In this study, the odorant receptor DmOr47a, the co-receptor DmOrco, and the calcium-sensing fluorescent protein GCaMP6f were stably expressed in a Pv11 cell line (Pv11-00443-Or47a), which is desiccatable. This cell line not only retained desiccation tolerance, but also showed dose-dependent fluorescence responses to the DmOr47a ligand pentyl acetate that were recovered 12&#xa0;h after rehydration. Even more importantly, Pv11-00443-Or47a showed a response to the agonist of DmOrco just 1&#xa0;h after rehydration, even upon inhibition of protein synthesis. This result demonstrates for the first time that a transmembrane protein can be dry-stored in an orthologous cell culture system. This work also constitutes an initial step towards the development of improved desiccatable sensing cells for use in portable devices.</p>

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An anhydrobiotic cell line expressing odorant receptors shows odorant responses after dry storage

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月10日

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  • Hiroto Fuse,
  • Richard Cornette,
  • Yugo Miyata,
  • Shoko Tokumoto,
  • Sachiko Shimura,
  • Ricardo C. Araneda,
  • Pamela Abshire,
  • Takahiro Kikawada,
  • Roy Anderson,
  • Redwan Haider,
  • Elisabeth Smela

摘要

Odorant receptor-expressing cells have been shown to recognize various odors, which has brought them to the interest of the growing field of cell-based olfactory sensors. However, cell cultures are difficult to use outside a laboratory because of their continuous need for controlled conditions. In this study, the odorant receptor DmOr47a, the co-receptor DmOrco, and the calcium-sensing fluorescent protein GCaMP6f were stably expressed in a Pv11 cell line (Pv11-00443-Or47a), which is desiccatable. This cell line not only retained desiccation tolerance, but also showed dose-dependent fluorescence responses to the DmOr47a ligand pentyl acetate that were recovered 12 h after rehydration. Even more importantly, Pv11-00443-Or47a showed a response to the agonist of DmOrco just 1 h after rehydration, even upon inhibition of protein synthesis. This result demonstrates for the first time that a transmembrane protein can be dry-stored in an orthologous cell culture system. This work also constitutes an initial step towards the development of improved desiccatable sensing cells for use in portable devices.