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<p><i>Kaempferia parviflora</i> (black ginger) is a tropical edible medicinal plant with valuable rhizome product used in the pharmaceutical industry for wide beneficial health effects in curing various diseases. The current study aims to analyse compounds present in rhizome oil using Gas Chromatography-Time of Flight-Mass Spectrometry (GC-TOF-MS). Hydro distillation was used to extract the essential oil from the plant rhizomes. 129 compounds were identified by GC TOF-MS analysis, with the main components being benzenepropanol, à-methyl-acetate (9.78 ± 0.01%), camphene (9.39 ± 0.01%), α-pinene (7.50 ± 0.01%), linalool (5.46 ± 0.01%), and camphore (5.04 ± 0.01%). Moreover, in this investigation, Benzenepropanol, à-methyl-, acetate, is Reported for the first time as a major constituent. The cytotoxic potential of the oil was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against a broad spectrum of human cancer cell lines, including the IC<sub>50</sub> value of A549 (38.73 ± 0.01&#xa0;µg/mL), A431 (68.33 ± 0.02&#xa0;µg/mL), HCT-15 (68.33 ± 0.01&#xa0;µg/mL), KB (88.57 ± 0.03&#xa0;µg/mL), LN-18 (43.23 ± 0.01&#xa0;µg/mL), Panc-1 (46.68 ± 0.01&#xa0;µg/mL), and SKBR-3(11.79 ± 0.01&#xa0;µg/mL). HEK293 (non-cancer cell line) was also included to check its cytotoxicity towards normal cells. Antimicrobial activities were assessed against <i>Staphylococcus aureus</i>, <i>Bacillus subtilis</i>, <i>Staphylococcus epidermidis</i>, <i>Klebsiella pneumoniae</i> and <i>Candida albicans</i> using disc diffusion method, showing highest zone of inhibition (ZOI) against <i>Klebsiella pneumoniae</i>,<i> Candida albicans</i> (30 ± 0.01&#xa0;mm) and the lowest against <i>Bacillus subtilis</i> (16 ± 0.02&#xa0;mm). The antioxidant activity revealed better IC<sub>50</sub> values in both 2,2-diphenyl-1-picrylhydrazyl (DPPH) as 0.50 ± 0.01&#xa0;mg/ml and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as 0.40 ± 0.01&#xa0;mg/ml assays. Hence, the obtained cytotoxic, antimicrobial and antioxidant potential of the tested oil, proved the potent quality of the <i>Kaempferia parviflora</i> oil. Hence, it can be used as an alternative antibiotic as well as antioxidant agent in the field of medicine and its commercialization in the global market.</p>

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Metabolic profile and bioactivity of the rhizome oil of Kaempferia parviflora from Eastern India

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  • 发表日期：

    2025年11月5日

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  • Rajashree Panigrahi,
  • Sameer Ranjan Sahoo,
  • Biswabhusan Dash,
  • Reena Parida

摘要

Kaempferia parviflora (black ginger) is a tropical edible medicinal plant with valuable rhizome product used in the pharmaceutical industry for wide beneficial health effects in curing various diseases. The current study aims to analyse compounds present in rhizome oil using Gas Chromatography-Time of Flight-Mass Spectrometry (GC-TOF-MS). Hydro distillation was used to extract the essential oil from the plant rhizomes. 129 compounds were identified by GC TOF-MS analysis, with the main components being benzenepropanol, à-methyl-acetate (9.78 ± 0.01%), camphene (9.39 ± 0.01%), α-pinene (7.50 ± 0.01%), linalool (5.46 ± 0.01%), and camphore (5.04 ± 0.01%). Moreover, in this investigation, Benzenepropanol, à-methyl-, acetate, is Reported for the first time as a major constituent. The cytotoxic potential of the oil was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against a broad spectrum of human cancer cell lines, including the IC50 value of A549 (38.73 ± 0.01 µg/mL), A431 (68.33 ± 0.02 µg/mL), HCT-15 (68.33 ± 0.01 µg/mL), KB (88.57 ± 0.03 µg/mL), LN-18 (43.23 ± 0.01 µg/mL), Panc-1 (46.68 ± 0.01 µg/mL), and SKBR-3(11.79 ± 0.01 µg/mL). HEK293 (non-cancer cell line) was also included to check its cytotoxicity towards normal cells. Antimicrobial activities were assessed against Staphylococcus aureus, Bacillus subtilis, Staphylococcus epidermidis, Klebsiella pneumoniae and Candida albicans using disc diffusion method, showing highest zone of inhibition (ZOI) against Klebsiella pneumoniae, Candida albicans (30 ± 0.01 mm) and the lowest against Bacillus subtilis (16 ± 0.02 mm). The antioxidant activity revealed better IC50 values in both 2,2-diphenyl-1-picrylhydrazyl (DPPH) as 0.50 ± 0.01 mg/ml and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as 0.40 ± 0.01 mg/ml assays. Hence, the obtained cytotoxic, antimicrobial and antioxidant potential of the tested oil, proved the potent quality of the Kaempferia parviflora oil. Hence, it can be used as an alternative antibiotic as well as antioxidant agent in the field of medicine and its commercialization in the global market.