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<p>Considering neo-angiogenesis as one of the most important hallmarks of solid tumors and a key promoter of recurrence, perfusion studies represent a modality to investigate tumor’s vascularization and to identify its influence on chemotherapy efficacy. To analyze neoplastic tissue adjacent to radiofrequency thermal-ablation (RFA) areas to demonstrate early perfusion anomalies and to suggest the synergic role of ablative techniques with chemotherapy treatments. In this prospective study, a total of 10 mice were subjected to a colon-carcinoma cell implantation and after the tumor colonization the RFA procedure was performed. All mice underwent a 7T MRI including 2D single-echo single-shot Dynamic-Contrast-Enhanced (DCE) sequences with intravenous injection of Gadolinium-based contrast agent (CA), before and after 72&#xa0;h from RFA treatment. Data-driven segmentation of the tumor mass enabled the identification of three ROIs with peculiar pharmacokinetic profiles of CA uptake: VTA, viable tumor area; PNA, partial necrotic area; CNA, complete necrotic area. Within these ROIs, permeability (K-trans and Extravascular-extracellular volume, Ve) and perfusion (Time to Peak, TTP) indices were evaluated with non-parametric paired samples Wilcoxon tests. Robust increase of Ve (PNA and VTA, <i>p</i> = 0.014, <i>p</i> &lt; 0.001, respectively) and TTP (VTA, <i>p</i> &lt; 0.01) is consistent with severe cellular and vascular damage induced by RFA. Perfusion MRI enables robust detection of significant modulation of the microvasculature within 72&#xa0;h from radiofrequency induced ablation in a murine model of colon-carcinoma. This opens a potentially new time window for the evaluation of therapeutic effects of RFA-enhanced chemotherapy.</p>

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7T MRI and histology reveal early tissue and perfusion changes after radiofrequency ablation in a murine colorectal cancer model

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月26日

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  • Mirko D’Onofrio,
  • Luisa Tomaiuolo,
  • Stefano Tambalo,
  • Diego Cavalli,
  • Beatrice Mascarin,
  • Marco Todesco,
  • Ilaria Mirandola,
  • Marco Gerosa,
  • Alessandro Negri,
  • Federica Vurro,
  • Flavia Merigo,
  • Nicolo’ Cardobi,
  • Riccardo De Robertis Lombardi,
  • Salvatore Paiella,
  • Pasquina Marzola,
  • Claudio Bassi

摘要

Considering neo-angiogenesis as one of the most important hallmarks of solid tumors and a key promoter of recurrence, perfusion studies represent a modality to investigate tumor’s vascularization and to identify its influence on chemotherapy efficacy. To analyze neoplastic tissue adjacent to radiofrequency thermal-ablation (RFA) areas to demonstrate early perfusion anomalies and to suggest the synergic role of ablative techniques with chemotherapy treatments. In this prospective study, a total of 10 mice were subjected to a colon-carcinoma cell implantation and after the tumor colonization the RFA procedure was performed. All mice underwent a 7T MRI including 2D single-echo single-shot Dynamic-Contrast-Enhanced (DCE) sequences with intravenous injection of Gadolinium-based contrast agent (CA), before and after 72 h from RFA treatment. Data-driven segmentation of the tumor mass enabled the identification of three ROIs with peculiar pharmacokinetic profiles of CA uptake: VTA, viable tumor area; PNA, partial necrotic area; CNA, complete necrotic area. Within these ROIs, permeability (K-trans and Extravascular-extracellular volume, Ve) and perfusion (Time to Peak, TTP) indices were evaluated with non-parametric paired samples Wilcoxon tests. Robust increase of Ve (PNA and VTA, p = 0.014, p < 0.001, respectively) and TTP (VTA, p < 0.01) is consistent with severe cellular and vascular damage induced by RFA. Perfusion MRI enables robust detection of significant modulation of the microvasculature within 72 h from radiofrequency induced ablation in a murine model of colon-carcinoma. This opens a potentially new time window for the evaluation of therapeutic effects of RFA-enhanced chemotherapy.