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<p>DNA repair is critical for cellular function and genomic stability across organisms. Yeast mating-type switching serves as an established model for studying DNA break repair and chromosome dynamics. However, real-time tracking of mating-type switching in live cells remains challenging due to resolution limitations of existing techniques. Here, we use high-throughput methods, including three-dimensional imaging, to follow the dynamics of DNA damage and repair and to quantify mating-type switching occurrences at the single live cell level, with unprecedented resolution. We reveal chromosome reconfiguration for both single- and double-strand breaks following switching induction. Our findings provide new observation of the correlation between chromosome folding and single-strand breaks.</p>

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High-throughput DNA repair monitoring in Saccharomyces cerevisiae suggests SSB- and DSB-induced chromatin reconfiguration

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年9月2日

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  • Yael Shalev Ezra,
  • Alon Saguy,
  • Gaia Levin,
  • Lucien E. Weiss,
  • Onit Alalouf,
  • Yoav Shechtman

摘要

DNA repair is critical for cellular function and genomic stability across organisms. Yeast mating-type switching serves as an established model for studying DNA break repair and chromosome dynamics. However, real-time tracking of mating-type switching in live cells remains challenging due to resolution limitations of existing techniques. Here, we use high-throughput methods, including three-dimensional imaging, to follow the dynamics of DNA damage and repair and to quantify mating-type switching occurrences at the single live cell level, with unprecedented resolution. We reveal chromosome reconfiguration for both single- and double-strand breaks following switching induction. Our findings provide new observation of the correlation between chromosome folding and single-strand breaks.