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<p>Chemical warfare between the host and the pathogen plays a crucial role in plant-necrotrophic pathogen interactions, but examples of its involvement in quantitative disease resistance in plants are poorly documented. In the <i>Daucus carota-Alternaria dauci</i> pathosystem, the novel toxin aldaulactone has been identified as a key factor in both fungal pathogenicity and the carrot’s partial resistance to the pathogen. Bioinformatic analyses have pinpointed a secondary metabolism gene cluster that harbors two polyketide synthase genes, <i>AdPKS7</i> and <i>AdPKS8</i>, that are likely responsible for the biosynthesis of aldaulactone. Here, we present the functional validation of <i>AdPKS7</i> and <i>AdPKS8</i> as genes responsible for aldaulactone production in <i>A. dauci</i>. We generated <i>A. dauci</i> knock-out mutants for <i>AdPKS7</i> and <i>AdPKS8</i> by replacing essential domains with a hygromycin resistance gene, marking the first reported case of genetic manipulation in <i>A. dauci</i>. Following transformation, the mutants were analyzed for toxin production via HPLC-UV and assessed for pathogenicity <i>in planta</i>. Aldaulactone production was abolished in all PKS mutants, which also exhibited significantly reduced pathogenicity on H1-susceptible carrot leaves. These findings confirm the roles of <i>AdPKS7</i> and <i>AdPKS8</i> in aldaulactone biosynthesis and their contribution to fungal pathogenicity.</p>

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Transformation of Alternaria dauci demonstrates the involvement of two polyketide synthase genes in aldaulactone production and fungal pathogenicity

  • 期刊论文

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  • 发表日期：

    2025年10月2日

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  • Jerome Monroe Bernardino,
  • Elza Neau,
  • Joséphine Kocuiba,
  • Maïwenn Gadras,
  • Amandine Rigaud,
  • Julia Courtial,
  • Jean-Jacques Helesbeux,
  • Dimitri Bréard,
  • Sophie Aligon,
  • Franck Bastide,
  • Bruno Hamon,
  • Justine Colou,
  • Pascal Poupard,
  • Nelly Bataillé-Simoneau,
  • Romain Berruyer

摘要

Chemical warfare between the host and the pathogen plays a crucial role in plant-necrotrophic pathogen interactions, but examples of its involvement in quantitative disease resistance in plants are poorly documented. In the Daucus carota-Alternaria dauci pathosystem, the novel toxin aldaulactone has been identified as a key factor in both fungal pathogenicity and the carrot’s partial resistance to the pathogen. Bioinformatic analyses have pinpointed a secondary metabolism gene cluster that harbors two polyketide synthase genes, AdPKS7 and AdPKS8, that are likely responsible for the biosynthesis of aldaulactone. Here, we present the functional validation of AdPKS7 and AdPKS8 as genes responsible for aldaulactone production in A. dauci. We generated A. dauci knock-out mutants for AdPKS7 and AdPKS8 by replacing essential domains with a hygromycin resistance gene, marking the first reported case of genetic manipulation in A. dauci. Following transformation, the mutants were analyzed for toxin production via HPLC-UV and assessed for pathogenicity in planta. Aldaulactone production was abolished in all PKS mutants, which also exhibited significantly reduced pathogenicity on H1-susceptible carrot leaves. These findings confirm the roles of AdPKS7 and AdPKS8 in aldaulactone biosynthesis and their contribution to fungal pathogenicity.