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<p>Porphyrinic MOF-based nanocomposites provide an applied attraction that overcomes the harmful effects of existing anticancer drugs. In this work, a nanorod porphyrinic metal-organic framework, PCN-222, was synthesized to serve as a high-surface-area drug carrier with photochemical properties. This material was then used for the production of Au nanoparticles. The PCN-222/Au/DOX NCs were provided with a low dosage of doxorubicin (DOX) as a novel nanocomposite with multifunctional anti-cancer properties on gastric cancer cells. The cytotoxicity effect was surveyed with an MTT assay on the MKN-45 cell line. The Annexin-V/propidium iodide technique and the colorimetric methods were utilized for apoptosis detection and caspase 8 and 9 pathway determination, respectively. The produced intracellular reactive oxygen species (ROS) were assigned using flow cytometry. Besides, the antibacterial activity of the nanocomposite was examined by conducting minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests on Helicobacter pylori under LED light and dark conditions. Achieving a 91% ratio of the apoptotic cells versus the total dead cells rate, the high ROS production with the main Florence intensity (MIF) value of about 247.5, and an approximately two-fold increase in caspase 9 compared to caspase 8, with a MIC value of 375&#xa0;µg/mL result under LED light for antibacterial activity, make the PCN-222/Au/DOX NCs an applicable promising agent with anticancer properties for gastric cancer cure.</p>

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Porphyrinic MOF-derived novel nanocomposite for gastric anticancer and Helicobacter pylori photoantibacterial effect assay

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月23日

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  • Sajedeh Tehrani Nejad,
  • Rahmatollah Rahimi,
  • Saeideh Eslaminejad

摘要

Porphyrinic MOF-based nanocomposites provide an applied attraction that overcomes the harmful effects of existing anticancer drugs. In this work, a nanorod porphyrinic metal-organic framework, PCN-222, was synthesized to serve as a high-surface-area drug carrier with photochemical properties. This material was then used for the production of Au nanoparticles. The PCN-222/Au/DOX NCs were provided with a low dosage of doxorubicin (DOX) as a novel nanocomposite with multifunctional anti-cancer properties on gastric cancer cells. The cytotoxicity effect was surveyed with an MTT assay on the MKN-45 cell line. The Annexin-V/propidium iodide technique and the colorimetric methods were utilized for apoptosis detection and caspase 8 and 9 pathway determination, respectively. The produced intracellular reactive oxygen species (ROS) were assigned using flow cytometry. Besides, the antibacterial activity of the nanocomposite was examined by conducting minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests on Helicobacter pylori under LED light and dark conditions. Achieving a 91% ratio of the apoptotic cells versus the total dead cells rate, the high ROS production with the main Florence intensity (MIF) value of about 247.5, and an approximately two-fold increase in caspase 9 compared to caspase 8, with a MIC value of 375 µg/mL result under LED light for antibacterial activity, make the PCN-222/Au/DOX NCs an applicable promising agent with anticancer properties for gastric cancer cure.