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<p>Plant growth–promoting rhizobacteria (PGPR) are soil microorganisms through which phytohormones and other bioactive compounds are produced, thereby enhancing plant growth and stress tolerance. In this study, a novel PGPR strain was identified from the rhizosphere of <i>Lycium chinense</i> seedlings, which produce protein-rich fruit. Whole-genome sequencing and annotation revealed that the genome of this strain, designated <i>Pseudomonas</i> sp. A-2, consists of a 6.65-Mb circular chromosome with 5,980 predicted protein-coding sequences. Comparative genomic analysis classified the strain within the genus <i>Pseudomonas</i>. The A-2 strain genome encodes proteins involved in indole-3-acetic acid (IAA) biosynthesis and signaling pathways, which was validated through IAA detection assays and quantitative analyses. Plant growth rates were significantly enhanced by the A-2 strain treatment, with increases of 3-fold in <i>Arabidopsis</i>, 1.5-fold in tobacco, and 1.35-fold in peanut. In <i>Arabidopsis thaliana</i>, expression of key genes associated with lateral and adventitious root formation was induced by the A-2 strain treatment, including <i>ARFs</i>, <i>AMI1</i>, <i>TAA1</i>, <i>YUCs</i>, <i>IBRs</i>, <i>TOB1</i>, and <i>ECH2</i>. Moreover, enhanced tolerance to salt stress was conferred by the A-2 strain treatment, as evidenced by improved biomass accumulation, chlorophyll content, antioxidant enzyme activity, and reduced lipid peroxidation. Levels of total soluble sugars, including trehalose, were elevated in the A-2 strain treated plants, suggesting a role in osmotic adjustment under stress. The plant growth–promoting and stress-alleviating properties of <i>Pseudomonas</i> sp. A-2 highlight its potential application as an effective biological agent for sustainable agriculture.</p>

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Functional characterization of a novel plant growth-promoting rhizobacterium enhancing root growth and salt stress tolerance

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月19日

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  • Sanghee Lee,
  • Young Kook Kim,
  • Hualin Nie,
  • Jongmin Ahn,
  • Nayoung Kim,
  • Seo-Rin Ko,
  • Ah-Hyeon Choi,
  • Hayoung Kwon,
  • Yuxin Peng,
  • Suk-Yoon Kwon,
  • Ah-Young Shin

摘要

Plant growth–promoting rhizobacteria (PGPR) are soil microorganisms through which phytohormones and other bioactive compounds are produced, thereby enhancing plant growth and stress tolerance. In this study, a novel PGPR strain was identified from the rhizosphere of Lycium chinense seedlings, which produce protein-rich fruit. Whole-genome sequencing and annotation revealed that the genome of this strain, designated Pseudomonas sp. A-2, consists of a 6.65-Mb circular chromosome with 5,980 predicted protein-coding sequences. Comparative genomic analysis classified the strain within the genus Pseudomonas. The A-2 strain genome encodes proteins involved in indole-3-acetic acid (IAA) biosynthesis and signaling pathways, which was validated through IAA detection assays and quantitative analyses. Plant growth rates were significantly enhanced by the A-2 strain treatment, with increases of 3-fold in Arabidopsis, 1.5-fold in tobacco, and 1.35-fold in peanut. In Arabidopsis thaliana, expression of key genes associated with lateral and adventitious root formation was induced by the A-2 strain treatment, including ARFs, AMI1, TAA1, YUCs, IBRs, TOB1, and ECH2. Moreover, enhanced tolerance to salt stress was conferred by the A-2 strain treatment, as evidenced by improved biomass accumulation, chlorophyll content, antioxidant enzyme activity, and reduced lipid peroxidation. Levels of total soluble sugars, including trehalose, were elevated in the A-2 strain treated plants, suggesting a role in osmotic adjustment under stress. The plant growth–promoting and stress-alleviating properties of Pseudomonas sp. A-2 highlight its potential application as an effective biological agent for sustainable agriculture.