Scholarlink

<p>Breast cancer is the most prevalent malignancy in women with 16% of all cancer deaths and current therapies frequently resulting in significant adverse consequences. Pumpkin seed oil (PSO) possesses antibacterial, antiviral, cytotoxic, and anticancer effects. Nonetheless, its hydrophobic characteristics restrict bioavailability, which can be enhanced by a nanoemulsion system. The optimization of pumpkin seed oil nanoemulsion (PSO-NE) was affected by surfactant concentration and sonication duration, resulting in a droplet size of 152.0&#xa0;nm, PDI of 0.189 and Zeta Potential of −&#xa0;23.5&#xa0;mV. PSO-NE displayed dose-dependent cytotoxicity, revealing a significantly decreased IC<sub>50</sub> compared to free PSO and Doxorubicin after 24&#xa0;h of exposure (p &lt; 0.01). At IC<sub>50</sub> concentration (MCF-7 = 46.6&#xa0;μg/ml and MDA-MB-231 = 60&#xa0;μg/ml) PSO-NE significantly elicited morphological alterations, decreased cell proliferation, enhanced phosphatidylserine exposure, and halted the cell cycle (p &lt; 0.001). The expression of genes (Bcl-2, STAT-3, and NF-kB), associated with cell proliferation, was significantly downregulated (p &lt; 0.001), while the expression of genes (p53, Bax, and caspase 3), linked to apoptosis, was significantly upregulated compared to the untreated cell lines (p &lt; 0.001), indicating the activation of apoptotic gene expression. The targeted cytotoxicity of PSO-NE against breast cancer and its ability to induce apoptosis and cell cycle arrest, underscores a promising anticancer drug.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

In-vitro assessment of anti-breast cancer potential of pumpkin seed oil nanoemulsion with mechanistic insights into cytotoxicity and apoptosis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月30日

查看全文
  • Rupali Ghosh,
  • Noor Fatima,
  • Abul Vafa,
  • Syed Abuzar Raza Rizvi,
  • Saima Wajid

摘要

Breast cancer is the most prevalent malignancy in women with 16% of all cancer deaths and current therapies frequently resulting in significant adverse consequences. Pumpkin seed oil (PSO) possesses antibacterial, antiviral, cytotoxic, and anticancer effects. Nonetheless, its hydrophobic characteristics restrict bioavailability, which can be enhanced by a nanoemulsion system. The optimization of pumpkin seed oil nanoemulsion (PSO-NE) was affected by surfactant concentration and sonication duration, resulting in a droplet size of 152.0 nm, PDI of 0.189 and Zeta Potential of − 23.5 mV. PSO-NE displayed dose-dependent cytotoxicity, revealing a significantly decreased IC50 compared to free PSO and Doxorubicin after 24 h of exposure (p < 0.01). At IC50 concentration (MCF-7 = 46.6 μg/ml and MDA-MB-231 = 60 μg/ml) PSO-NE significantly elicited morphological alterations, decreased cell proliferation, enhanced phosphatidylserine exposure, and halted the cell cycle (p < 0.001). The expression of genes (Bcl-2, STAT-3, and NF-kB), associated with cell proliferation, was significantly downregulated (p < 0.001), while the expression of genes (p53, Bax, and caspase 3), linked to apoptosis, was significantly upregulated compared to the untreated cell lines (p < 0.001), indicating the activation of apoptotic gene expression. The targeted cytotoxicity of PSO-NE against breast cancer and its ability to induce apoptosis and cell cycle arrest, underscores a promising anticancer drug.