Scholarlink

<p><i>Colletotrichum humuli</i> was originally described from diseased leaves of <i>Humulus lupulus</i> collected in 1890. Due to the absence of molecular data, an isotype specimen was examined to clarify its systematic position. The nuclear ribosomal ITS2 region of this specimen was successfully amplified and sequenced using the primer ITS4 along with a newly designed primer, ITSCF1. Additional gene sequences (<i>GAPDH</i>, <i>CHS-1</i>, <i>HIS3</i>, and <i>ACT</i>) were also retrieved, marking the first successful recovery of multiple loci from an ancient <i>Colletotrichum</i> herbarium specimen. Multi-locus phylogenetic analyses demonstrated that <i>C. humuli</i> is genetically distinct from all other known species. Furthermore, comparison with isolates from <i>H. scandens</i> in South Korea revealed a novel species, described here as <i>C. humulicola</i>; together, these two species form a new species complex within the genus <i>Colletotrichum</i>. This study provides a straightforward protocol for obtaining DNA sequences from historical herbarium material.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Multi-locus study of Colletotrichum humuli from an ancient herbarium specimen and description of C. humulicola sp. nov. from Humulus scandens

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月22日

查看全文
  • Le Dinh Thao,
  • Hyeon-Dong Shin,
  • Hyorim Choi,
  • Donghun Kang,
  • Anbazhagan Mageswari,
  • Jae Sung Lee,
  • Daseul Lee,
  • In-Young Choi,
  • Ulrike Damm,
  • Seung-Beom Hong

摘要

Colletotrichum humuli was originally described from diseased leaves of Humulus lupulus collected in 1890. Due to the absence of molecular data, an isotype specimen was examined to clarify its systematic position. The nuclear ribosomal ITS2 region of this specimen was successfully amplified and sequenced using the primer ITS4 along with a newly designed primer, ITSCF1. Additional gene sequences (GAPDH, CHS-1, HIS3, and ACT) were also retrieved, marking the first successful recovery of multiple loci from an ancient Colletotrichum herbarium specimen. Multi-locus phylogenetic analyses demonstrated that C. humuli is genetically distinct from all other known species. Furthermore, comparison with isolates from H. scandens in South Korea revealed a novel species, described here as C. humulicola; together, these two species form a new species complex within the genus Colletotrichum. This study provides a straightforward protocol for obtaining DNA sequences from historical herbarium material.