Scholarlink

<p>Colorectal cancer is the third most common cancer. Anticancer compounds obtained after the digestion of snail eggs may hurt the growth and development of colon cancer cells. The research aimed to prepare hydrolyzates from <i>Helix aspersa maxima</i> and <i>Helix aspersa aspersa</i> snail eggs using static <i>in vitro</i> simulation of gastrointestinal digestion. Redox status indicators, concentration of total protein, total carbohydrates, uronic acids, allantoin, and glycolic acid, and electrophoretic profile of proteins and glycoproteins were compared in hydrolyzates and non-digested extracts. The effect of hydrolyzates on the integrity of the plasma membrane of Caco-2 human epithelial colorectal adenocarcinoma cells and the monolayer of IEC-6 rat intestinal cells was determined. The influence of hydrolyzates on the production of reactive oxygen species (ROS) by Caco-2 cells, the potential of their mitochondrial membrane, and the production of apoptotic proteins were examined. Incubation with hydrolyzates for 72&#xa0;h reduced the plasma membrane integrity of Caco-2 cells but not normal cells, did not affect ROS content, decreased mitochondrial membrane potential (the tendency for <i>H. a. aspersa</i> hydrolyzate), and altered the concentration of some apoptotic proteins of intrinsic and extrinsic apoptosis pathways. Bioactive compounds released during digestion and their interactions may have been responsible for anticancer effects.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Toxic effects of Helix aspersa snail egg hydrolyzates obtained by static in vitro digestion on Caco-2 colorectal adenocarcinoma cells

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月25日

查看全文
  • Magdalena Matusiewicz,
  • Joanna Kuczka,
  • Michalina Danił,
  • Klara Piotrowska,
  • Hanna Antushevich,
  • Tomasz Niemiec

摘要

Colorectal cancer is the third most common cancer. Anticancer compounds obtained after the digestion of snail eggs may hurt the growth and development of colon cancer cells. The research aimed to prepare hydrolyzates from Helix aspersa maxima and Helix aspersa aspersa snail eggs using static in vitro simulation of gastrointestinal digestion. Redox status indicators, concentration of total protein, total carbohydrates, uronic acids, allantoin, and glycolic acid, and electrophoretic profile of proteins and glycoproteins were compared in hydrolyzates and non-digested extracts. The effect of hydrolyzates on the integrity of the plasma membrane of Caco-2 human epithelial colorectal adenocarcinoma cells and the monolayer of IEC-6 rat intestinal cells was determined. The influence of hydrolyzates on the production of reactive oxygen species (ROS) by Caco-2 cells, the potential of their mitochondrial membrane, and the production of apoptotic proteins were examined. Incubation with hydrolyzates for 72 h reduced the plasma membrane integrity of Caco-2 cells but not normal cells, did not affect ROS content, decreased mitochondrial membrane potential (the tendency for H. a. aspersa hydrolyzate), and altered the concentration of some apoptotic proteins of intrinsic and extrinsic apoptosis pathways. Bioactive compounds released during digestion and their interactions may have been responsible for anticancer effects.