Fabrication of a low-kink-radius bilayer vascular scaffold incorporating a TPU stent fabricated via melt electrowriting and an electrospun PCL/PU/gelatin layer
摘要
This study investigates the fabrication of a small-diameter bilayer vascular graft, which is an inner layer fabricated from melt-electrowriting (MEW) thermoplastic polyurethane (TPU) scaffold and an outer co-electrospun layer made of heparinized polycaprolactone (PCL)/polyurethane (PU)/gelatin, aimed at mimicking the extracellular matrix (ECM). The bilayer structure exhibited good flexibility, mechanical stability, and anti-thrombogenic properties, overcoming the drawbacks of vascular grafts, such as high kink radius and tendency toward thrombosis. MTT assays proved cytocompatibility, showing an increase in cell proliferation over 7 days, the optical density of the bilayer vascular graft increased from