<p>Amphipods have elicited interest for use as a live feed to culture marine species. However few studies have been conducted to determine optimal conditions necessary for large scale culture. The amphipod, <i>Parhyale hawaiensis</i> is a tropical species that has been considered for use in warm water marine culture. A 30 days study examined the effects of culture system and diet on amphipod production. Two culture systems (biofloc, clearwater) and three diets (fresh and dried <i>Ulva lactuca</i>, commercial shrimp diet) were compared. Water quality, and total adult, juvenile and hatchling densities were evaluated. Water quality was not significantly different between treatments, although a trend towards lower ammonia and nitrite levels were seen in biofloc systems. After 30 days, 50% of the population was comprised of juveniles, with adults and hatchlings each comprising 25% of the population. Total amphipod density differed significantly between treatments with highest density in the biofloc + fresh <i>U. lactuca</i> treatment and lowest density in the clear water + fresh <i>U. lactuca</i> treatment. Significant differences were seen in adult and juvenile populations, with higher densities noted in biofloc systems. Although no significant differences were seen with regards to diet, amphipods fed the <i>U. lactuca</i> diets showed more variation in density than those fed the commercial shrimp diet. Results of this study suggest that utilization of a biofloc culture system may both improve water quality and increase <i>P. hawaiensis</i> production. It is further suggested that more consistent production may been seen if amphipods are fed a commercial shrimp diet rather than a <i>U. lactuca</i> diet.</p>