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<p><i>Staphylococcus epidermidis</i> is an opportunistic commensal cutaneous biofilm-producing agent frequently causing musculoskeletal infections (MSI) with/without implants. This study was design to evaluate phenotypic and genomic relatedness between commensal skin and MSI <i>S. epidermidis</i> isolates and to assess patient-related and microbial markers in the outcome of patients after one-year follow-up. Demographics, clinical data and monomicrobial <i>S. epidermidis</i> isolates of MSI patients (<i>n</i> = 31) and healthy individuals (<i>n</i> = 15) were analyzed. Phenotypic profile was assessed by susceptibility tests by broth microdilution and biofilm formation. Phylogenetic relationships, resistome characterization, and virulome analysis were carried out by complete genome sequencing (<i>n</i> = 46). Overall, MSI-derived isolates were significantly more strong/moderate biofilm producers and depicted higher rates of resistance to methicillin (MRSE), ciprofloxacin, gentamicin and rifampicin. Demographics and clinical characteristics did not significantly affect MSI patients’ outcomes. In the whole-genomic sequencing (WGS) phylogeny, most MSI-derived isolates were grouped into the pathogenic-associated clonal complex CC2, and significantly higher prevalence of <i>mec</i>A gene and pathogenic marker IS256. Following multivariate analysis, MSI-derived isolates carrying transposable element IS256 are more likely to develop a persistent infection (odds ratio [OR], 8.00, [95% confidence interval (CI), 1.06 to 60.31], <i>P</i> = 0.044), while weaker biofilm producer were protectors (OR, 0.070, 95%CI, 0.005–0.979, <i>P</i> = 0.048), reducing the chance of recurrence by 93% (1–0.070). The logistic regression model’s performance was evaluated by Nagelkerke’s R, which resulted in 47.21%. In conclusion, <i>S. epidermidis</i> isolates producing MSI were phenotypically and genetically distinct from commensals, proven the association between independent genetic traits and patient’s outcome.</p>

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Comparative genomic analysis of resistance and virulence genes in staphylococcus epidermidis and their impact on clinical outcome of musculoskeletal infections

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月2日

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  • Ingrid Nayara Marcelino Santos,
  • Felipe Alberto-Lei,
  • Fernanda Fernandes Santos,
  • Mariana Felix Cerqueira Balera,
  • Mariana Neri Kurihara,
  • Carolina Coelho Cunha,
  • Laís Sales Seriacopi,
  • Thomas Stravinskas Durigon,
  • Fernando Baldy Reis,
  • Ana Karolina Antunes Eisen,
  • Giovana Santos Caleiro,
  • Jansen de Araújo,
  • Edison Luiz Durigon,
  • Giselle Burlamaqui Klautau,
  • Mauro José Salles

摘要

Staphylococcus epidermidis is an opportunistic commensal cutaneous biofilm-producing agent frequently causing musculoskeletal infections (MSI) with/without implants. This study was design to evaluate phenotypic and genomic relatedness between commensal skin and MSI S. epidermidis isolates and to assess patient-related and microbial markers in the outcome of patients after one-year follow-up. Demographics, clinical data and monomicrobial S. epidermidis isolates of MSI patients (n = 31) and healthy individuals (n = 15) were analyzed. Phenotypic profile was assessed by susceptibility tests by broth microdilution and biofilm formation. Phylogenetic relationships, resistome characterization, and virulome analysis were carried out by complete genome sequencing (n = 46). Overall, MSI-derived isolates were significantly more strong/moderate biofilm producers and depicted higher rates of resistance to methicillin (MRSE), ciprofloxacin, gentamicin and rifampicin. Demographics and clinical characteristics did not significantly affect MSI patients’ outcomes. In the whole-genomic sequencing (WGS) phylogeny, most MSI-derived isolates were grouped into the pathogenic-associated clonal complex CC2, and significantly higher prevalence of mecA gene and pathogenic marker IS256. Following multivariate analysis, MSI-derived isolates carrying transposable element IS256 are more likely to develop a persistent infection (odds ratio [OR], 8.00, [95% confidence interval (CI), 1.06 to 60.31], P = 0.044), while weaker biofilm producer were protectors (OR, 0.070, 95%CI, 0.005–0.979, P = 0.048), reducing the chance of recurrence by 93% (1–0.070). The logistic regression model’s performance was evaluated by Nagelkerke’s R, which resulted in 47.21%. In conclusion, S. epidermidis isolates producing MSI were phenotypically and genetically distinct from commensals, proven the association between independent genetic traits and patient’s outcome.