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<p>Microplastics (MPs) are a common long-lasting pollutant of aquatic ecosystems. Microalgae are primary producers of aquatic systems, and MP contamination could have a high impact on the aquatic food web. Therefore, the present study utilized polyethylene (PE) particles (0 to 150&#xa0;mg/L) for investigating the half-maximal inhibitory concentrations (IC<sub>50</sub>) of <i>Chlorella sorokiniana</i> and also studied their impacts on growth rate, biomass, pigments and other biochemical components of the microalgae. After 96&#xa0;h of incubation, PE of 100&#xa0;mg/L resulted in the half-maximum inhibition (IC<sub>50</sub>). After reaching the stationary phase (14 d), harvesting was made for MP-exposed cultures to reveal a biomass production of 0.89&#xa0;g/L, while it was 0.96&#xa0;g/L for the control. A slight reduction in pigment and lipid contents was also observed, while the protein and carbohydrate contents were high in MP-exposed <i>C. sorokiniana</i> cells. Under the MP stress, reactive oxygen species (ROS) and phenolic levels were reduced, whereas flavonoid content increased. PE particles were characterized using Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) and Fourier Transform infrared spectroscopy (FT-IR) for their size, shape, chemical composition, and interaction with <i>C. sorokiniana</i>, followed by micro-Fourier Transform infrared spectroscopy (µ-FT-IR) for the mapping of MP. This research contributes to a deeper understanding of how MP contamination can disrupt aquatic food webs, guiding future ecological assessments and pollution management strategies.</p>

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Exploring biochemical responses and cellular adaptations of Chlorella sorokiniana to polyethylene microplastic exposure

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月2日

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  • Jyoti Rawat,
  • Pooja Bhatnagar,
  • Abhilasha Mishra,
  • Manisha Nanda,
  • Prateek Gururani,
  • Afzal Hussain,
  • Mikhail S. Vlaskin,
  • Sanjay Kumar,
  • Monu Verma,
  • Hyunook Kim,
  • Vinod Kumar

摘要

Microplastics (MPs) are a common long-lasting pollutant of aquatic ecosystems. Microalgae are primary producers of aquatic systems, and MP contamination could have a high impact on the aquatic food web. Therefore, the present study utilized polyethylene (PE) particles (0 to 150 mg/L) for investigating the half-maximal inhibitory concentrations (IC50) of Chlorella sorokiniana and also studied their impacts on growth rate, biomass, pigments and other biochemical components of the microalgae. After 96 h of incubation, PE of 100 mg/L resulted in the half-maximum inhibition (IC50). After reaching the stationary phase (14 d), harvesting was made for MP-exposed cultures to reveal a biomass production of 0.89 g/L, while it was 0.96 g/L for the control. A slight reduction in pigment and lipid contents was also observed, while the protein and carbohydrate contents were high in MP-exposed C. sorokiniana cells. Under the MP stress, reactive oxygen species (ROS) and phenolic levels were reduced, whereas flavonoid content increased. PE particles were characterized using Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDX) and Fourier Transform infrared spectroscopy (FT-IR) for their size, shape, chemical composition, and interaction with C. sorokiniana, followed by micro-Fourier Transform infrared spectroscopy (µ-FT-IR) for the mapping of MP. This research contributes to a deeper understanding of how MP contamination can disrupt aquatic food webs, guiding future ecological assessments and pollution management strategies.